缺氧诱导因子-1α及血管生长因子-C在直肠癌组织中的表达及其意义  

The expression and significance of hypoxia inducible factor-1α and vascular endothelial growth factor-C in colorectal cancer

作　　者：朱小康[1] 韩福生[2] 李兴文[1] 刘先富[2] 

机构地区：[1]甘肃省肿瘤医院肿瘤外科,甘肃兰州733000 [2]蚌埠医学院第一附属医院肿瘤外科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2011年第12期1299-1302,共4页Journal of Bengbu Medical College

摘　　要：目的:检测缺氧诱导因子-1α(HIF-1α)与血管生长因子-C(VEGF-C)在直肠癌中的表达,分析其与直肠癌临床病理参数的关系,探讨其在直肠癌的发生和淋巴转移过程中的作用。方法:采用免疫组织化学EliVsion plus法检测72例直肠癌组织中HIF-1α、VEGF-C的表达及VEGFR-3染色的微淋巴管密度。结果:HIF-1α、VEGF-C在直肠癌及癌旁组织中阳性率分别为77.8%、83.0%和44.4%、42.0%,差异均有统计学意义(P<0.01);直肠癌淋巴结转移者中HIF-1α阳性率为90.0%,无转移者为63.5%,两者差异有统计学意义(χ2=7.78,P<0.01);直肠癌淋巴结转移者中VEGF-C阳性率为95.0%,无转移者为68.8%,两者差异有统计学意义(χ2=8.82,P<0.01)。在直肠癌高、中分化组和低、未分化组HIF-1α阳性率分别为12%和44%,差异有统计学意义(P<0.01);VEGF-C阳性率分别为14%和46%,差异有统计学意义(P<0.01);HIF-1α与VEGF-C在直肠癌组织中的表达呈相关关系(r=0.105,P<0.01)。结论:HIF-1α、VEGF-C在直肠癌患者癌组织中高表达,在癌旁组织中低表达,且其阳性表达与直肠癌淋巴结转移及癌组织分化程度有关,与年龄、性别无关。HIF-1α、VEGF-C在直肠癌组织中表达具有相关性,两者在直肠癌浸润转移过程中可存在协同效应。Objective：To investigate the expression of hypoxia inducible factor-1α（HIF-1α） and vascular endothelial growth factor-C（VEGF-C） and their relation to lymphangiogenesis and lymphatic metastasis in colorectal cancer.Methods：The expression of HIF-1α and VEGF-C in colorectal cancer tissues and normal colorectal tissues were detected in 72 patients with immunohistochemical EliVsion plus method and microlymphtic vessel density in VEGFR-3 staining was calculated.Results：The positive rates of HIF-1α and VEGF-C expressions density in colorectal cancer and adjacent normal tissue were 77.8%,83.0% and 44.4%,42.0%,respectively,there were the statistical differences（P0.01）.The positive rate of HIF-1α was 90.0% in lymph node metastasis cases,no transfer for 63.5%,the difference had statistical significance（P0.01）.The positive rate of VEGF-C was 95.0% in lymph node metastasis cases,no transfer for 68.8%,which had the statistical difference（χ2=7.78,P0.01）;the positive rates of HIF-1α were 12% and 44%,which had the statistical significance（χ2=8.82,P0.01）.In high,medium differentiation of colorectal cancer group and low,undifferentiated group,the positive rates of VEGF-C was 14% and 46%,respectively,also had the statistical difference（P0.01）.The expression of HIF-1α and VEGF-C in colorectal cancer tissues had synergistic effect（r=0.105,P0.01）.Conclusions：The expression of HIF-1α and VEGF-C are high in colorectal cancer,and low in adjacent tissues.The positive expression is associated with lymph node metastasis and the differentiation degree in carcinoma tissues,and has no relationship with age and sex.The expression of HIF-1α and VEGF-C are associated with each other in colorectal cancer tissues,and exists the synergy effect in colorectal cancer metastasis process.

关 键 词：直肠肿瘤 缺氧诱导因子-1Α 血管生成因子-C 免疫组织化学 

分 类 号：R735.37[医药卫生—肿瘤]