乌贼墨对H_(22)癌细胞内Ca^(2+)浓度、核膜Ca^(2+)/Mg^(2+)-ATP酶活性及c-jun表达的影响  

Effect of sepia on intracellular Ca^( 2+) concentration,nuclear Ca^(2+) /Mg^( 2+)-ATPase activities and c-jun expression in H_22 cancer cells

作　　者：王成斌[1] 孙克任[2] 谢克勤[2] 苏小游 

机构地区：[1]中国预防医学科学院环境卫生监测所,北京100021 [2]山东医科大学山东省毒理学重点实验室

出　　处：《卫生研究》2000年第2期96-98,共3页Journal of Hygiene Research

基　　金：山东省卫生厅科学基金资助!( No.90 C0 83 0 )

摘　　要：利用荧光探针和免疫组织化学方法,研究了乌贼墨对H22癌细胞内Ca2+浓度、核膜Ca2+/Mg2+-ATP酶活性及c-jun表达的影响。结果发现,乌贼墨使H22癌细胞内Ca2+浓度分别降低了69%和79%,核膜Ca2+/Mg2+-ATP酶活性分别降低了21%和37%,c-jun表达明显减少。结果提示乌贼墨可能通过降低细胞内Ca2+浓度,继而影响到核膜上Ca2+依赖性Ca2+/Mg2+-ATP酶活性,减少了Ca2+向细胞核内的转运,使Ca2+对c-jun表达的促进作用减弱,抑制了细胞的分裂增殖。这可能是乌贼墨抑制肿瘤生长的机制之一。The effect of sepia on the intracellular Ca 2+ concentration,nuclear Ca 2+ /Mg 2+ ATPase activities and the expression of c jun were studied in H 22 cancer cells by using fluorescent probe and immunohistochemical method.The results showed that intracellular Ca 2+ concentration decreased 69% and 79%,nuclear Ca 2+ /Mg 2+ ATPase activities diminished 21% and 37%,c jun expression decreased obviously.The results suggested that sepia probably diminished the intracellular Ca 2+ concentration,affected Ca 2+ dependent nuclear Ca 2+ /Mg 2+ ATPase activities,thus reduced the amount of Ca 2+ transporting into nuclei,so lessened the promoting effect of Ca 2+ on c jun expression,and therefore inhibited cellular differentiation and proliferation.This might be one of the possible anti cancer mechanisms of sepia.

关 键 词：乌贼墨 肿瘤 抑制作用 H22癌细胞 钙 

分 类 号：R73-36[医药卫生—肿瘤]