通脑灵对NMDA介导神经细胞内游离钙离子浓度的影响  

EFFECTS OF TONGANOLING ON AUGMENTATION OF CYTOPLASMIC FREE CALCIUM CONCENTRATION IN NEURAL CELLS INDUCED BY NMDA

作　　者：刘卫红[1] 邵念方[2] 高英茂[3] 

机构地区：[1]山东中医药大学 [2]山东中医药大学附属医院,山东济南250011 [3]山东医科大学,山东济南250012

出　　处：《山东中医药大学学报》2000年第2期150-151,共2页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省自然科学基金! ( Y96C0 5 0 3 7)

摘　　要：目的 :研究通脑灵对 N-甲基 - D-门冬氨酸 (NMDA)介导的神经细胞内游离钙离子 ([Ca2 + ]i)浓度的影响。方法 :应用标记示踪法 ,以 Fura- 2 / AM为荧光指示剂观察通脑灵对 NMDA介导的神经细胞内游离钙离子浓度的影响。结果 :5 0 0μm ol· L- 1 NMDA可使神经细胞内 [Ca2 + ]i明显增高 ,与对照组比较有显著性差异 (P<0 .0 1)。通脑灵可阻止 NMDA介导的神经细胞内 [Ca2 + ]i超载。结论 :通脑灵通过抑制Objective:To study effects of Tongnaoling on augmentation of cytoplasmic free calcium concentration in neural cells induced by NMDA.Methods:Cytoplasmic free calcium concentrations in neral cells induced by NMDA were determined and those treated with Tongnaoling by Fura 2/AM fluorescene technique.Results: [Ca 2+ ]i was elevated,which is higher than that in control group(P<0.01).Tongnaoling inhibited the increase of [Ca 2+ ]i induced by NMDA.Conclsion:Tongnaoling protect neural cells through inhibited increase of [Ca 2+ ]i induced by NMDA effectively. [KEY WORDS] Tongnaoling;NMDA;calcium;neural cells;Fura 2/AMg in an inflammatory hyperalgesia. Key words Inflammatory hyperalgesia; DRG; I h channel; Action potentialery and the prognosis of hilar cholangiocarcinoma.Methods We studied retrospectively 58 cases of hilar cholangiocarcinoma which entered our department between June 1987 and October 1998. The postoperative liver functional recovery and mortality and morbidity between unilateral ( n =27) and bilateral ( n =31) bile duct drainage groups were compared.Results The liver function in bilateral drainage group was nearly normal within 6 weeks after operation. The ALb level of unilateral drainaged patients recovered gradually to normal after operation, and the TBIL and ALT decrease nearly to the normal range within 6 weeks after operation. The AKP decreased within 2 postoperative weeks, then steadily increased. The differences of perioperative complication rate and mortality of the two groups showed no significance. Conclusion The data showed that the liver function can recover to some extent by unilateral bile duct drainage, and unilateral drainage operations are the choice for hilar cholangiocarcinoma that can not be excised now.

关 键 词：通脑灵 NMDA 神经细胞 钙离子 

分 类 号：R285.5[医药卫生—中药学]