长心卡帕藻(Kappaphycus alvarezii)表达序列标记分析  

ANALYSIS OF KAPPAPHYCUS ALVAREZII EXPRESSED SEQUENCE TAGS

作　　者：刘晨临[1] 黄晓航[1] 刘建国[2] 

机构地区：[1]国家海洋局第一海洋研究所海洋生态研究中心,青岛266061 [2]中国科学院海洋研究所,青岛266071

出　　处：《海洋与湖沼》2011年第6期804-810,共7页Oceanologia Et Limnologia Sinica

基　　金：国家海洋公益项目;2012418039号;200705010号;国家海洋局基本科研业务经费项目;2008G19号

摘　　要：分别采用BlastX和Blast2go软件对453条非冗余的长心卡帕藻EST序列进行匹配分析和GO注释。结果表明,有281条序列与蛋白数据库中序列匹配,有132条序列被归属为3个子本体:分子功能、生物学过程和细胞组成。同时筛选到24个与逆境适应相关的基因,包括应对胁迫反应和抗氧化反应的基因。采用GCUA软件,进行长心卡帕藻基因密码子偏倚性的研究。结果表明其密码子偏倚性与其它红藻差别较大,平均G+C含量和密码子的第三位的G+C含量均较其它藻类偏低。上述工作为进一步研究长心卡帕藻基因及其功能奠定了基础。There were 281 sequences found significantly similar to the known proteins using the protein database BlastX aligning of 453 Kappaphycus alvarezii TUGs. GO annotation was conducted on 453 TUGs using Blast2go, 132 sequences were assigned to have molecular function, biological process and/or cellular component sub-ontology. According to the results of GO annotation, 24 genes were found associated with stress adaptation, including genes respond to stimulus and genes involve in antioxidant activity. Codon usage of K. alvarezii was also analyzed based on the 23251 codons from these 132 known genes. The codon usage bias was found quite different to the other red algae. The average GC content in codons and the GC usage in the third codon position were lower than the other algae. These data have laid a foundation for further study on gene functions ofK. alvarezii. Key words Kappaphycus alvarezii,

关 键 词：长心卡帕藻 表达序列标记分析 抗逆基因 密码子使用频率 

分 类 号：Q93[生物学—微生物学]