同型半胱氨酸致内皮细胞、平滑肌细胞和泡沫细胞低密度脂蛋白受体DNA甲基化的比较研究  

Comparison of endothelial cells,vascular smooth muscle cells and foam cells induced by LDLR DNA methylation in homocysteine

作　　者：孙炜炜[1] 马琳娜[1] 徐支芳[2] 马长剑[1] 王菊[1] 巩慧慧[1] 姜怡邓[1] 

机构地区：[1]宁夏医科大学检验学院,宁夏银川750004 [2]宁夏医科大学基础医学院病理生理学教研室,宁夏银川750004

出　　处：《中国现代医学杂志》2011年第35期4355-4358,4362,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金[No:30960124(2009);No:81160044(2011)];宁夏自然科学基金(No:NZ1086)

摘　　要：目的观察同型半胱氨酸(Hcy)对内皮细胞、平滑肌细胞以及泡沫细胞中低密度脂蛋白受体(LDLR)DNA甲基化的影响。方法原代培养内皮细胞、人脐静脉血管平滑肌细胞(VSMCs)和单核细胞源性泡沫细胞,用0、50、100、200和500μM Hcy干预后培养72 h,提取基因组DNA,巢式降落式PCR(nMS-PCR)法分别检测3种细胞中LDLR DNA甲基化修饰状态的变化,以观察Hcy对不同细胞LDLR的作用和影响。结果内皮细胞、VSMCs与不同浓度Hcy培养72 h后,LDLR基因启动子区DNA呈高甲基化改变,泡沫细胞LDLR启动子区DNA呈低甲基化改变,与对照组比较差异有统计学意义(P<0.05,P<0.01),且以50或100μM Hcy组最明显。结论 Hcy对不同细胞LDLR启动子区DNA甲基化的影响有差异性,提示可能存在更深层次的机制。[ Objective ] To observe the efftct of low density lipoprotein receptor （LDLR） DNA methylation induced endothelial cells, vascular smooth muscle cells （VSMCs） and foam cells in homocysteine （Hcy）. [Methods] 0, 50, 100, 200, 500 p,M concentrations of Hcy were added into the primary cultured endothelial eeUs, VSMCs and monocyte-derived foam eells for 72 h. Detected the LDLR methylation status changes of three different cells by nMS-PCR, and to observe the efftct of Hey on them. [Results] Endothelial cells, VSMCs cultured with different concentrations of Hey after 72 h, LDLR gene showed DNA homomethylation while the foam ceils showed DNA hypomethylated. Compared with the eontrol group, there were significaot differences （P〈0.05, P〈0.01）, obviously the 50 μM or the 100 μM group. [Conclusion] The effect of Hey on LDLR promoter DNA methylation are different, suggesting that there may be deeper mechanisms.

关 键 词：低密度脂蛋白受体 DNA甲基化 同型半胱氨酸 

分 类 号：R363[医药卫生—病理学]