胆总管腺癌Musashi-1和ALDH1表达及其临床病理意义  

Expression of Mus-1 and ALDH1 and its significance in the adenocarcinoma of common bile duct

作　　者：黄江生[1] 

机构地区：[1]中南大学湘雅二医院,湖南长沙410011

出　　处：《中国现代医学杂志》2011年第35期4409-4413,共5页China Journal of Modern Medicine

摘　　要：目的研究胆总管腺癌和癌旁组织中Musashi-1和ALDH1两种癌干细胞标记物表达水平及其临床病理意义。方法 40例胆总管腺癌和15例癌旁组织手术切除标本常规制作石蜡包埋切片,PUMA和ABCG2染色方法为EnVisionTM免疫组织化学法。结果胆总管腺癌Mus-1和ALDH1表达阳性率明显高于癌旁组织(Mus-1:52.5%与13.3%,χ2=6.89,P<0.01;ALDH1:40.0%与6.7%,χ2=5.68,P<0.05);PUMA和ABCG2表达阳性的癌旁组织上皮均呈现轻至中度不典型增生;腺癌Ⅰ级和淋巴结未转移病例Mus-1和ALDH1表达阳性率低于腺癌Ⅲ级和淋巴结转移病例(P<0.05);Mus-1和ALDH1在胆总管腺癌中的表达呈明显一致性(χ2=6.19,P<0.05)。结论 Mus-1和ALDH1表达与胆总管腺癌发生、临床生物学行为及预后有较密切的关系。[Objective] To study the expressive levels of Mus-1 and ALDH1 and their clinicopathologieal significances in the adenocareinoma and its peritumoral tissues of common bile duct. [Methods] EnVisionTM immunohistochemistry for detecting expressive levels of Mus-1 and ALDH1 was used in the conventional paraffin-embedded sections from the specimens of adenocareinoma of common bile duct （n =40）, and peritumoral tissues （n =15）. [Results] The positive rates of Mus-1 and ALDH1 were significantly higher in adencarcinoma than that peritumoral tissues （Mus-1： 52.5% vs 13.3%, X^2=6.89, P〈0.01; ALDHI： 40.0% vs 6.7%, X^2=5.68, P 〈0.05）. The peritumoral tissues of Mus-1 and ALDH1 positive expresson of showed atypical-hyperplasia of epitheli. The positive rates of Mus-1 and ALDH1 were significantly lower in the cases of histologic grade l, the cases of maximal diameter 42 cm and no-metastasis of lymph node than those in the ones of histologic grade lll, the ones of maximal diameter 〉2 cm and metastasis of lymph node in adenocarcinoma of common bile duct （P 〈0.05 or P 〈0.01）. The closely positive correlation was found between the scores of Mus-1 and ALDH1 in adenocarcinoma of common bile duct （Xz=6.19, P〈0.05）. [Conclusions] The expression of Mus-1 and ALDH1 might be closely related to the carcinogenesis, clinical biological behaviors and prognosis in adenocarcinoma of common bile duct.

关 键 词：胆总管肿瘤 Musashi家族蛋白 乙醛脱氢酶1 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]