急性白血病患者抑癌基因p27的表达及其启动子甲基化状态的研究  

Research on expression and promoter methylation of tumor suppressor gene p27 in acute leukemia

作　　者：汤宏宇 何勤[2] 王秀琴[3] 孙学蓉 张利娟[2] 朱鸿斌[2] 游崇登[2] 

机构地区：[1]六盘水市人民医院,贵州六盘水553000 [2]昆明医学院第二附属医院 [3]秦皇岛市第一医院

出　　处：《山东医药》2012年第4期38-40,共3页Shandong Medical Journal

基　　金：昆明医学院研究生创新基金项目(KM2008L17)

摘　　要：目的研究急性白血病(AL)患者抑癌基因p27的表达及其启动子的甲基化状态,探讨其在AL发生发展中的作用。方法采用逆转录—聚合酶链反应(RT-PCR)及甲基化特异性聚合酶链反应(MSP)技术研究AL患者骨髓单个核细胞p27 mRNA的表达水平及其启动子甲基化发生率。结果 AL组和对照组p27 mRNA阳性率分别为36.9%和87.8%(P<0.01),p27 mRNA的表达缺失与AL患者外周血白细胞计数、髓外浸润呈负相关(P均<0.05)。AL患者的p27基因启动子甲基化阳性率较低(4.62%)。结论 p27基因的表达缺失是AL发生发展的机制之一,p27基因启动子甲基化是否为AL患者p27基因表达缺失的机制之一尚需进一步研究证实。Objective To investigate the changes of p27 gene expression and promoter methylation status in acute leukemia（AL） patients,as well as explore their roles in the development of AL and the relationship that exists between them.Method Reverse transcription-polymerase chain reaction（RT-PCR） and methylation-specific polymerase chain reaction（MSP） technology were used to research the mRNA expression level and promoter methylation of p27 gene in bone marrow mononuclear cells of AL patients.Results p27 mRNA-positive rates in AL group and the control group were 36.9% and 87.8%（P0.01）,p27 mRNA expression in peripheral blood of patients with AL loss of white blood cell count,extramedullary infiltration was negatively correlated（P0.05）.p27 gene promoter methylation-positive rate of AL patients was low（4.62%）.Conclusion Loss of expression of p27 gene is one of the mechanisms of the development AL,therefore,p27 gene promoter methylation is one of the mechanisms,whether induces p27 gene expression deletion or not still need further research.

关 键 词：急性白血病 P27基因 DNA甲基化 

分 类 号：R733.7[医药卫生—肿瘤]