CD44v6与人乳头瘤病毒在宫颈鳞癌及癌前病变中的相关性研究  

Expression of CD44v6 in the Cervical Intraepithelial Neoplasia and Cervical Squamous Cell Carcinoma with HPV Infection

作　　者：卢佳[1] 杨永秀[2] 高丽[2] 杨爱宏[2] 

机构地区：[1]兰州大学第一临床医学院,730000 [2]兰州大学第一医院妇产科

出　　处：《国际妇产科学杂志》2012年第1期100-102,I0002,共4页Journal of International Obstetrics and Gynecology

摘　　要：目的:观察CD44v6与人乳头瘤病毒(HPV)在宫颈鳞癌及癌前病变中的相关性,以探讨两者联合检测在宫颈癌早期诊断中的意义。方法:采用免疫组化(SP)法检测宫颈鳞癌、宫颈上皮内瘤变(CIN)Ⅰ、CINⅡ、CINⅢ及正常宫颈组织各20例石蜡标本中CD44v6的表达,同时用荧光定量聚合酶链反应(FQ-PCR)法检测上述组织中HPV-DNA的相对含量,将两者检测的结果进行统计学分析并研究其相关性。结果:CD44v6在对照组、CINⅠ组、CINⅡ组、CINⅢ组及宫颈鳞癌组中细胞表达的阳性率逐渐升高,5组比较差异有统计学意义(P<0.05);HPV感染率也逐渐升高,5组比较差异有统计学意义(P<0.05);CD44v6与HPV感染呈正相关(rs=0.341,P=0.01)。结论 :CD44v6的异常表达和HPV感染与宫颈癌的发生、发展关系密切,两者联合检测对宫颈癌的早期诊断有一定的临床意义。Objective：To investigate the relationship between expression of CD44v6 and HPV infection in cervical intraepithelial neoplasia and cervical squamous cell carcinoma.Methods：Using Streptavidin-Peroxidase（S-P） immunohistochemical technique,the authors examined the expression of CD44v6 in samples.The infection of HPV-DNA was determined by FQ-PCR.Results：There were significant difference for CD44v6 expression between cervical squamous cell carcinoma,CIN and normal tissues.（P〈0.05）.The positive rate of HPV was significant different among cervical squamous cell carcinoma,CIN and normal tissues（P〈0.05）.The infection of HPV was associated with the expression of CD44v6（rs=0.341,P=0.01）.Conclusions：The abnormal expression of CD44v6 and HPV infection are closely related with the early pathogenesis,development of cervical cancer.So there is of certain clinical significance for early diagnosis of cervical cancer by detecting CD44v6 and HPV.

关 键 词：抗原 CD44 宫颈肿瘤 免疫组织化学 癌前状态 聚合酶链反应 

分 类 号：R737.33[医药卫生—肿瘤]