抵抗素基因-420C>G位点多态性对脑出血患者血浆抵抗素水平的影响  

The Effect of-420C>G Polymorphism of Resistin Gene on Plasma Resistin Concentrations in the Patients with Intracerebral Hemorrhage

作　　者：董晓巧[1] 俞文华[1] 张祖勇[1] 陈隽 翁建丰 朱强[1] 车志豪[1] 杜权[1] 

机构地区：[1]浙江杭州市第一人民医院,310006 [2]浙江慈溪市第二人民医院,315315

出　　处：《心脑血管病防治》2012年第1期18-20,共3页CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT

基　　金：杭州市医药卫生科技计划项目(2010A009)

摘　　要：目的探讨抵抗素基因-420C>G位点单核苷酸多态性对脑出血患者血浆抵抗素水平的影响。方法选取344例高血压性基底核出血患者,入院时抽取外周血提取DNA,采用聚合酶反应-限制性片断多态性分析基因型,同时采用ELISA法检测血浆抵抗素浓度,进行统计分析。结果酶切后可见抵抗素基因-420C>G位点有CC、CG和GG三种基因型,等位基因C和G的频率分别为71.08%和28.92%,基因型CC、CG和GG的频率依次为50.29%、41.57%和8.14%。经Hardy-Weinberg遗传平衡定律检验,各基因型频率符合遗传平衡。CC、CG和GG基因型患者血浆抵抗素浓度依次为(23.83±7.09)ng/mL、(26.54±7.32)ng/mL和(27.18±9.97)ng/mL。CC基因型患者血浆抵抗素均显著低于CG和GG基因型患者(P<0.01,P<0.05)。结论抵抗素基因-420C>G位点单核苷酸多态性显著影响脑出血患者血浆抵抗素水平,可能参与脑出血炎症反应。Objective This study aimed to discuss the effect of the -420C 〉 G single nucleotide polymorphism of resistin gene on the plasma resistin concentrations in the patients with intracerebral hemorrhage. Methods 344 patients with intracerebral hemorrhage were included in this study. Plasma resistin concentrations were measured and -420C 〉 G polymorphism of resistin gene was genotyped us- ing PCR-restriction fragment length polymorphism, then made statistical analysis. Results The genotypes of the -420C 〉 G polymor- phism of resistin gene included CC, CG and GG , the C and G allele frequencies were 71.08% and 28.92%, the genotype CC, CG and GG frequencies were 50.29%, 41.57% and 8.14%, as well as the genotype frequencies of the -420C 〉 G polymorphism of re- sistin gene were in the Hardy-Weinberg equilibrium. Plasma resistin levels of the patients with the genotype CC, CG and GG were （23.83 ＋ 7.09）ng/mL, （26.54 ＋ 7.32）ng/mL and （27.18 ＋ 9.97）ng/mL respectively. The patients with genotype C-C had lower resistin compared with those with genotype CG and GG （ P 〈 0.01, P 〈 0.05）. Conclusions The -420C 〉 G single nucleotide poly- morphism of resistin gene may affect plasma resistin concentrations and seemed to be potentially involved in the inflammatory compo- nent of intracerebral hemorrhage.

关 键 词：抵抗素 基因多态性 脑出血 炎症反应 

分 类 号：R743.3[医药卫生—神经病学与精神病学]