抑制复制蛋白A1表达对食管癌细胞放射敏感性的影响  

The effect of inhibiting the expression of replication protein AI on radiosensitivity of human esopha. Ileal carcinoma cells

作　　者：顾玉明[1] 王洵[1] 辛勇 章龙珍 许伟[1] 裴冬生 郑骏年 

机构地区：[1]徐州医学院附属医院介入放射科,221000 [2]徐州医学彘附属医院介入放射治疗科,221000 [3]江苏省肿瘤生物治疗重点实验室

出　　处：《中华实验外科杂志》2012年第3期478-480,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察抑制复制蛋白A1（RPA1）表达对食管癌细胞放射敏感性的影响。方法脂质体介导转染RPA1反义寡核苷酸及阴性对照寡核苷酸于人食管癌TE．1细胞中，设空白对照组（只加培养基）、Mock组（转染试剂组）、对照组（阴性对照寡核苷酸组）及实验组（反义寡核苷酸组），分别以24、48、72、96h为转染时间点；通过逆转录．聚合酶链反应（RT—PCR）、实时荧光定量PCR法（qRT—PCR）和Westernblot法分别检测各组食管癌TE-1细胞RPAlmRNA及其蛋白表达；并给予6MVX射线单次照射6Gy，用细胞计数试剂盒（CCK）-8法检测各组食管癌细胞放射敏感性的差异。结果lit．PCR、qRT-PCR及Westernblot法结果显示实验组食管癌TE-1细胞的RPAlmRNA及蛋白表达均降低；CCK-8结果显示实验组食管癌TE-1细胞的增殖率低于其他各组（P〈0．05）。结论抑制食管癌TE-1细胞RPAl表达，可增加食管癌TE-1细胞的放射敏感性。Objective To investigate the effect of knocking down the expression of replication pro- tein A1 （RPA1） on radiosensitivity of human esophageal carcinoma cells. Methods Antisense oligonu- leotides of RPA1, mediated by LipofectamineTM 2000, were transfected into TE-1 cells. Four groups were set up： blank control group, mock group （only LipofectamineTM 2000 ）, negative control group and anti- sense transfected group at 24, 48, 72, 96 b. Tge expression levels of RPA1 mRNA in TE-1 cells were de- termined by using reverse transeription-polymerase chain reaction （RT-PCR） and quantitative real-time PCR （ qRT-PCR）, and those of RPA1 pmtain by using Western blotting. TE-1 ceils were irradiated with 6 MV X-ray at the dose of 6 Gy. Cell counting Kit-8 （CCK-8） method was used to analyze the radiosensitivi- ty of TE-1 ceils after tansfected with antisense oligonuleotides of RPA1. Results RPA1 mRNA and protein were down-regulated in antisense transfected group as compared with other groups. CCK-8 method revealed that antisense oligonuleotides of RPA1 could improve the radiosensitivity of TE-1 ceUs as compared with other groups （P 〈 0. 05 ）. Conclusion Antisense oligonuleotides of RPA1 can induce the down-regulation of RPA1 in esophageal carcinoma cells, and improve the radiosensitivity of esophageal carcinoma cells.

关 键 词：食管癌 复制蛋白A1 放射敏感性 

分 类 号：R735.1[医药卫生—肿瘤]