单纯性肺泡癌和含有肺泡癌成分的腺癌基因差异比较  

Differentially expressed genes between pure bronchioloalveolar carcinoma and pure bronchioloalve- olar carcinoma features by genechip

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作  者:张文成[1] 岳东升[1] 张连民[1] 张真发[1] 王长利[1] 

机构地区:[1]天津市肿瘤防治重点实验室天津医科大学附属肿瘤医院肺部肿瘤科,300060

出  处:《中华实验外科杂志》2012年第3期484-487,共4页Chinese Journal of Experimental Surgery

基  金:国家自然科学基金资助项目(30801377)

摘  要:目的比较单纯性肺泡癌(PureBAC)与含有肺泡癌成分的腺癌(AWBF)的基因表达谱,研究与PureBAC进展相关的基因。方法选取PureBAC标本8例,以8例AWBF作对照。采用Agiilent 人4*44Karrays全基因组芯片进行检测,应用DAVID、通路studi05.0、特征提取软件9.1等软件对基因芯片的结果进行统计学、聚类和差异表达分析。选取两组间差异表达的43个侵袭和转移的基因(P〈0.01),采用Real—time聚合酶链反应(PCR)验证基因芯片结果。应用SPSS统计软件分析。结果表达谱芯片共得到581个基因的有效数据,筛选出43个侵袭和转移的基因(P〈0.01),差异表达上调基因23个,下调基因20个。实时定量RT—PCR检测结果显示ATF-2基因的相对表达量单纯性BAC组明显高于AWBF组,而CLDN18基因在AWBF组明显增高(P〈0.01),而且CLDN18和AT2在BAC和AWBF中的基因表达差异倍数〉10倍。结论CLDN18和ATF2在BAC中和AWBF中基因表达有明显差异,两者的异常表达可能与单纯性BAC的进展有关。Objective To investigate the genetic differences between pure bronchioloalveolar car- cinoma (BAC) and invasive mixed type adenocarcinoma with BAC features (AWBF) and to explore the molecular mechanism of pure BAC progression. Methods Total RNA was extract froml6 lung tissue speci- mens, ineludig 8 cases of BAC and 8 cases of AWBF. The BAC and AWBF were analyzed for gene expres- sion profiles using the Agiilent 4 * 44K arrays. Differentially expressed candidate genes were validated u- sing quantitative real-time polymerase chain reaction (PCR). The SPSS software was used for the analysis. Two-sided values of P were given, and statistical signifcance was set at P 〈 0. 01. Results We selected 43 genes correlated with tumor progression and metastasis. A total of 23 genes were significantly higher in the AWBF and 20 genes were lower. Of the 43 validated genes, CLDN18 and ATF-2 were found to have 〉 10 fold higher expression in AWBF ( P 〈 0. 01 ) than in BAC. Conclusion CLDN18 and ATF-2 may be related with the progression of BAC to AWBF. Furthermore, targeting these genes may be a promising way for AWBF treatment.

关 键 词:肺泡癌 差异表达 基因表达 

分 类 号:R734.2[医药卫生—肿瘤]

 

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