TAT—GluR6—9C与pMON原核表达载体重组体的构建  

Constructing prokaryotic expression recombinant pMON- TAT- GIuR6 -9C

作　　者：胡书群[1] 刘东海[1] 张光毅[1] 

机构地区：[1]江苏省脑病生物信息重点实验室,徐州医学院生物化学与分子生物学研究中心,江苏徐州221002

出　　处：《徐州医学院学报》2011年第12期788-791,共4页Acta Academiae Medicinae Xuzhou

基　　金：基金项目：国家自然科学基金面上项目（90608015）;江苏省自然科学基金面上项目（BK2010171）;江苏省高校自然科学基金重点项目（10KJA310053）;徐州医学院院长基金（2010KJZl2）

摘　　要：目的表达和纯化TAT—GluR6—9C小肽，用于脑缺血细胞信号转导的研究。方法用化学合成的方法获得TAT—GluR6—9C小肽的cDNA；与原核表达载体pMON用T4DNA连接酶连接；连接产物转化大肠杆菌JMl09进行筛选、序列测定。结果①经化学合成并退火得到了TAT—GluR6—9C的双链cDNA；②酶切鉴定能观察到TAT—GluR6—9C和pMON两条带；③TAT—GluR6—9C测序图谱与GenBank报道完全一致。结论获得了含目的基因TAT—GluR6—9C的pMON原核表达载体重组体。Objective To express and purify the TAT - GluR6 - 9C for the study of the cell signal transduction after ischemic brain. Methods The cDNA of TAT- GluR6 -9C was synthetized by chemical method; then, the cDNA was subsequently cloned into the prokaryotic expression vector pMON ; lastly, the recombinant was transformed into E coll. JM109 and the sequence were determined. Results （1）The double - chain cDNA of TAT - GluR6 - 9C was synthetized by chemical method and annealed. （2）The recombinant contains two bands of pMON/TAT - GluR6 - 9C by restriction enzyme digestion. （3）The sequence of cloned TAT - GluR6 - 9C is in accordence with GenBank. Conclusion Recombi- nant of pMON - TAT - GluR6 - 9C is obtained.

关 键 词：TAT—GluR6—9C pMON原核表达载体 序列测定 

分 类 号：R743.31[医药卫生—神经病学与精神病学]