艾迪协同依托泊苷诱导骨肉瘤细胞凋亡的实验研究  

Apoptosis of osteosarcoma cells induced by combination of AiDi and etoposide

作　　者：黄涛[1] 李旭辉[2] 邹春平[1] 李修成[1] 冯殿鹏[1] 

机构地区：[1]中国医科大学附属第一医院骨科,辽宁沈阳110001 [2]清华大学生命科学学院,北京100084

出　　处：《中国现代医学杂志》2011年第30期3758-3762,共5页China Journal of Modern Medicine

基　　金：辽宁省教育厅高等学校科技计划(No:L2010655)

摘　　要：目的研究中药制剂艾迪对骨肉瘤化疗的增效作用及其相关机制。方法将艾迪与依托泊苷单独及联合应用于骨肉瘤OS732细胞,用MTT法检测细胞抑制率,流式细胞仪定量分析凋亡细胞所占比例,倒置相差显微镜及荧光显微镜下观察凋亡细胞形态改变,免疫细胞化学技术分析凋亡相关蛋白Fas表达。结果艾迪与依托泊苷对骨肉瘤细胞都有剂量依赖性杀伤作用,艾迪浓度达到25μl/mL时,细胞抑制率为38.47%,再增加艾迪浓度抑制率不再明显改变,但如与1μg/mL依托泊苷合用将使细胞抑制率进一步提升为51.62%(P<0.01)。细胞超微结构观察及凋亡率测定也显示,艾迪与依托泊苷联用可诱导更多骨肉瘤细胞凋亡,且联用时骨肉瘤Fas表达明显提升(P<0.01)。结论艾迪与小剂量依托泊苷合用与单用依托泊苷相比可更高效杀伤骨肉瘤细胞,此作用与上调Fas表达诱导骨肉瘤细胞凋亡有关。[ Objective ] To study the apoptosis of osteosarcoma ceils induced by combination of AiDi and etoposide and related mechanism. [Methods] AiDi and etoposide were applied on the osteosarcoma cells respectively or jointly and inhibition rate was measured by MT？ assay. The cellular ultrastructure were observed with inverted phase contrast microscope and fluorescence microscope; The apoptotic rate were analyzed with flow cytometry （FCM）. Immunoeytochemistry were used to examine the expression of Fas on 0S732 cells. [ Results ] Both AiDi and etoposide has the kilting effect on OS732 cell lines depending on the drug doze. The inhibition rate was 38.47% when 25μ/ml AiDi was used on OS732 cell lines and the inhibition rate did not rise significantly with more AiDi application, if combined with 1μg/mL etoposide. However, inhibition rate was 51.62% which was significantly higher than that of 25μl/mL AiDi individually （P 〈0.01）. Changes of cellular ultrastructure and apoptotic rate also indicated apoptosisinducing effect of joint application was much stronger than those of respective application ,in addition, up-regulated expression of Fas and induced apoptosis were also observed along with the killing effect of OS732 cell lines （P 〈 0.01）. [ Conclusion] Combination of AiDi and small doze of etoposide has stronger killing effect on OS732 cell lines compared with the individual application of etoposide which may be initiated by up-regulated Fas expression and induced apoptosis.

关 键 词：艾迪 依托泊苷 骨肉瘤 凋亡 

分 类 号：R738.1[医药卫生—肿瘤]