茶树细胞周期蛋白依赖激酶(CsCDK)基因cDNA全长克隆与分析  

cDNA Cloning and Expression Analysis of Cyclin-dependent Kinase(CsCDK) Gene in Tea Plant

作　　者：王新超[1] 马春雷[1] 杨亚军[1] 金基强[1] 马建强[1] 曹红利[1] 

机构地区：[1]中国农业科学院茶叶研究所/国家茶树改良中心,杭州310008

出　　处：《园艺学报》2012年第2期333-342,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30872059);现代农业(茶叶)产业技术体系绿茶育种岗位项目(CARS-23)

摘　　要：采用SMART-RACE-PCR技术获得了茶树细胞周期蛋白依赖激酶基因(CsCDK1)的全长cDNA序列,并进行了相关的生物信息学分析。采用实时定量PCR研究了该基因在茶树越冬芽休眠到萌发后不同阶段的表达模式。克隆到茶树CsCDK1cDNA序列1245bp(GenBank登录号JF449383),其开放阅读框长924bp,编码307个氨基酸,预测分子量为34.52kD,具有CDKs家族典型的保守结构域和空间结构,属于B型CDK家族。系统进化分析表明,CsCDK1氨基酸序列与猕猴桃的CDK亲缘关系最近,达到96%,与其它植物的CDK序列相似性亦在80%以上。该基因在茶树越冬芽休眠期的表达量低于恢复生长期,在萌发期表达量最高。说明该基因与茶树越冬芽休眠的解除关系密切。The full length cDNA sequence of cyclin-dependent kinase gene was cloned by SMARTRACE-PCR from tea plan（tCamellia sinensis L.）,which was named as CsCDK1（GenBank Accession No.JF449383）.Its expression pattern during the time of bud dormancy and bud break in tea plant was analyzed by quantitative real-time PCR（qRT-PCR）.The full length cDNA of CsCDK1 is 1 245 bp,which includes 924 bp complete CDS and encodes 307 amino acid residues with a putative molecular mass of 34.52 kD.The bioinformatics characterization indicated that the protein encoded by CsCDK1 gene has a typically conserved domain and 3D structure of B type CDK family,and it had the highest identity（96%） with CDK of Actinidia chinensis,and it shared over 80%amino acid sequences similarity with CDKs of other plants.The results of quantitative real-time PCR showed that the expression levels of CsCDK1 at dormant stages were lower than growth stages,and the highest expression lever appeared at sprouting stage.It indicates that CsCDK1 has very close relationship with tea plant budbreak in spring.

关 键 词：茶树 芽 萌发 细胞周期蛋白依赖激酶基因 表达分析 

分 类 号：S571.1[农业科学—茶叶生产加工]