当归补血汤对动脉粥样硬化兔内皮祖细胞及血清VEGF、SDF-1的影响  

Effects of Danggui Buxue decoction on endothelial progenitor cells,serum VEGF and SDF-1 in atherosclerotic rabbits

作　　者：秦臻[1] 黄水清[1] 

机构地区：[1]广州中医药大学,广东广州510405

出　　处：《中国病理生理杂志》2012年第2期211-215,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30973895)

摘　　要：目的:观察当归补血汤对兔动脉粥样硬化模型外周血内皮祖细胞(endothelial progenitor cells,EPCs)功能及血清血管内皮生长因子(vascular endothelial growth factor,VEGF)、基质细胞衍生因子1(stromal cell-derived factor 1,SDF-1)表达的影响。方法:新西兰兔25只,免疫损伤结合高脂饮食法建立动脉粥样硬化模型,模型动物随机分为5组,每组5只。模型组灌胃等量蒸馏水,辛伐他汀组灌胃辛伐他汀水悬液1.7 mg/kg,当归补血汤高、中、低剂量组分别灌胃当归补血汤6 g/kg、3 g/kg、1.5 g/kg,每天1次,2周后取兔血检测VEGF和SDF-1水平,培养并鉴定兔外周血EPCs,MTT法检测增殖能力,黏附实验检测黏附能力,Transwell小室检测迁移能力,体外血管生成试剂盒检测形成小管能力。结果:与模型组比较,辛伐他汀及当归补血汤高、中剂量组家兔血清VEGF和SDF-1水平增高(P<0.05),且EPCs的增殖、黏附、迁移和形成小管能力均有增强(P<0.05)。结论:当归补血汤可能通过提高循环VEGF和SDF-1水平来促进动脉粥样硬化EPCs的活性。AIM： To investigate the effects of Danggui Buxue decoction（DBD） on serum vascular endothelial growth factor（VEGF） and stromal cell-derived factor 1（SDF-1）,as well as the activity of circulating endothelial progenitor cells（EPCs） in atherosclerotic rabbits.METHODS： The model of atherosclerosis was established using immune injury and fatty diet for 4 weeks in New Zealand rabbits（n=25）.All modeled rabbits were randomized into 5 groups with 5 animals in each group.The rabbits in atherosclerosis group were intragastrically administered with distilled water.The rabbits in simvastatin group were treated with simvastatin at the dose of 1.7 mg/kg.The rabbits in DBD high-dose,middle-dose and low-dose treatment groups were given DBD at the doses of 6 g/kg,3 g/kg and 1.5 g/kg,respectively.All drugs were given once a day for 2 weeks.After treatment,the levels of serum VEGF and SDF-1 were measured.The mononuclear cells isolated from the rabbit peripheral blood were cultured for 7 days in vitro,and then attached cells were cultured with both DiI-ac-LDL and FITC-UEA-1 for identification.The proliferation was detected by MTT method.The cell migration was observed using Transwell chambers.The adhesion determination and in vitro angiogenesis assay were also performed.RESULTS： Compared with atherosclerosis group,the levels of serum VEGF and SDF-1 were elevated（P0.05）,the proliferation,migration,adhesion and angiogenesis of EPCs were all improved in DBD high-dose,middle-dose treatment groups and simvastatin group（P0.05）.CONCLUSION： DBD elevates the levels of serum VEGF and SDF-1 to improve the activity of EPCs in the process of atherosclerosis.

关 键 词：当归补血汤 内皮祖细胞 动脉粥样硬化 血管内皮生长因子 基质细胞源性因子1 

分 类 号：R363[医药卫生—病理学]