干扰素诱导蛋白p204表达变化对大鼠血管平滑肌细胞增殖及p21表达的影响  

Effects of p204 expression on expression of p21 and proliferation of vascular smooth muscle cells in rats

作　　者：龙向淑[1] 吴强[1] 宋方[1] 

机构地区：[1]贵州省人民医院心血管内科,贵州贵阳550002

出　　处：《中国病理生理杂志》2012年第2期249-252,共4页Chinese Journal of Pathophysiology

基　　金：贵州省优秀科技教育人才省长专项资金资助项目(No.黔省专合字[2009]30号)

摘　　要：目的:观察干扰素诱导蛋白p204对鼠血管平滑肌细胞(VSMCs)增殖及p21表达的影响,探讨p204在VSMCs增殖调控中的作用及可能机制。方法:应用干扰素α(IFN-α)处理和p204基因(Ifi204)的小干扰RNA(siRNA)瞬时干预体外培养的VSMCs,用MTT法测定细胞活力反映细胞增殖,流式细胞仪分析细胞周期,用半定量RT-PCR法和Western blotting分别检测p204和p21的mRNA和蛋白表达。结果:IFN-α可诱导鼠VSMCsp204 mRNA和蛋白表达上调,降低VSMCs细胞活力和细胞周期G1/S转换,伴p21 mRNA和蛋白表达上调。转染Ifi204 siRNA可抑制p204和p21表达,提高VSMCs细胞活力和促进细胞周期G1/S转换。结论:p204表达可抑制鼠VSMCs的增殖,该效应可能与激活p21表达有关。AIM： To observe the effect of interferon-inducible protein 204（p204） on the expression of p21 and proliferation of vascular smooth muscle cells（VSMCs） in rats.METHODS： Interferon alpha（IFN-α） and small interference RNA（siRNA） targeting p204 gene（Ifi204） was used to intervene cultured VSMCs in vitro instantaneously,then the cell vitality was determined by MTT assay to reflect the cell proliferation.The cell cycle was analyzed by flow cytometry.The expression of p204 and p21 at mRNA and protein levels was determined by semi-quantitative RT-PCR and Western blotting.RESULTS： In rat VSMCs,IFN-α induced the increase in the expression of p204 at mRNA and protein levels,reduced the cell vitality and the G1/S phase transition,and up-regulated the expression of p21 at mRNA and protein levels.Transfection of Ifi204 siRNA restrained the expression of p204 and p21,increased the cell vitality and promoted the G1/S phase transition.CONCLUSION： The expression of p204 restrains the proliferation of rat VSMCs,probably by activating the expression of p21.

关 键 词：干扰素 干扰素诱导蛋白 p204 血管平滑肌细胞 小干扰RNA 

分 类 号：R363[医药卫生—病理学]