SBP1在大肠癌中的表达及临床病理相关性分析  

The related clinicopathologic analysis of SBP1 expression in colorectal carcinoma

作　　者：李晓[1] 孙自勤[1] 魏志[1] 刘晓峰[1] 汪伟[1] 贾爱芹[1] 

机构地区：[1]中国人民解放军济南军区总医院消化科,山东济南250031

出　　处：《胃肠病学和肝病学杂志》2012年第2期140-143,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：济南军区总医院院长基金(一等)(2010.12.1-2015.12.1)

摘　　要：目的研究硒结合蛋白1(SBP1)在大肠癌组织中的表达及其临床病理学意义。方法采用免疫组化方法检测25例大肠癌、大肠腺瘤、癌旁组织中SBP1表达及细胞定位,比较大肠癌、癌旁组织、大肠腺瘤中SBP1的阳性率;采用RT-PCR检测20例大肠癌及癌旁组织,10例大肠腺瘤中SBP1的水平,比较大肠癌、癌旁组织、大肠腺瘤中SBP1 mRNA相对表达量,分析大肠癌组织中SBP1表达水平与各临床参数之间的关系。结果大肠癌组织、大肠腺瘤组织及癌旁正常大肠组织中SBP1表达阳性率分别为40%、72%及88%,大肠癌中SBP1表达率显著低于大肠腺瘤组织和癌旁大肠组织(P<0.05)。SBP1在大肠癌中的表达与组织分化程度有关(P<0.05),与其他临床病理因素无关(P>0.05)。结论 SBP1可能抑制大肠癌的进展,我们的研究结果可能为今后的SBP1在肿瘤发生的分子机制,以及大肠癌的化学预防和治疗中作用的研究提供一个基础。Objective To explore the expression of SBP1 and clinical pathological significance in colorectal earcinoma.Methods Expression of SBP1 mRNA in adjacent corresponding colorectal tissues,colorectal carcinoma and colorectal adenoma were evaluated by immunohistochemistry and RT-PCR.The relationship between expression of SBP1 in colorectal adenoma and clinical parameters was analyzed.Results There was negative expression of SBP1 in colorectal carcinoma.The positive expression rate of SBP1 was 72%（18/25） in colorectal adenoma and 88%（22/25） in adjacent corresponding colorectal tissues.Compared with colorectal adenoma and adjacent tissues,expression of SBP1 in colorectal carcinoma obviously decreased（P0.05）.The expression of SBP1 was positively correlated with histopathobigical grades（P0.05）,but not with other clinicopathological factors（P0.05）.Conclusion The low expression of SBP1 may be associated with carcinogenesis an progression of colorectal carcinoma.Our findings may provide a basis for future studies concerning the molecular mechanism of SBP1 in tumorigenesis as well as a possible role of selenium in chemoprevention and treatment of colorectal carcinoma.

关 键 词：大肠癌 硒结合蛋白1 免疫组化学 实时定量逆转录聚合酶链反应 

分 类 号：R735.34[医药卫生—肿瘤]