神经生长因子与表皮生长因子干预创伤性脑损伤后内源性神经干细胞的增殖  

Effect of nerve growth factor and epidermal growth factor on proliferation of endogenous neural stem cells after traumatic brain injury

作　　者：刘阳[1] 刘卫平[2] 王孝安[2] 杨扬[2] 龙乾发[2] 郑朝晖[2] 张玉奇[2] 钟俊[2] 

机构地区：[1]绵阳市第三人民医院神经外科,四川省绵阳市621000 [2]解放军第四军医大学西京医院神经外科,陕西省西安市710032

出　　处：《中国组织工程研究与临床康复》2012年第1期65-69,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：四川省卫生厅课题资金资助(2010-100301)~~

摘　　要：背景:表皮生长因子、神经生长因子可促进神经干细胞增殖,但对创伤性脑损伤后成年大鼠脑内内源性干细胞的影响研究甚少。目的:观察创伤性脑损伤后神经生长因子和表皮生长因子对内源性神经干细胞增殖的影响。方法:采用改良Feeney氏法自由落体撞击的方法建立大鼠创伤性脑损伤模型,48只大鼠随机抽签法分为4组,外伤后24h分别行神经生长因子、表皮生长因子单独或联合注射,对照组注射等量生理盐水。结果与结论:神经生长因子组、表皮生长因子组和联合组前肢放置实验及平衡实验评分均优于对照组,联合组优于单独治疗组(P<0.05)。单独治疗组和联合组大鼠室管膜下区、海马和损伤区域出现的BrdU阳性细胞数明显多于对照组,联合治疗组多于单独治疗组(P<0.05)。提示创伤性脑损伤后使用神经生长因子、表皮生长因子均可增加大鼠模型内源性神经干细胞的增殖和肢体功能的恢复,而且两种神经营养因子联合应用使这种效果更为明显。BACKGROUND： Epidermal growth factor （EGF） and nerve growth factor （NGF） can promote the proliferation of neural stem cells, but their effects on endogenous stem cells after a traumatic brain injury in adult rats are studied little. OBJECTIVE： To investigate the effect of NGF and EGF on proliferation of endogenous neural stem cells after traumatic brain injury in rats. METHODS： Forty-eight adult SD rats were divided into three treatment groups and one control group at random, and the traumatic brain injury model was established with improved Feeney’s free-fall impacting method. Separate and combined injection of NGF and EGF were given to rats at 24 hours after traumatic brain injury. Normal saline was administrated into the rats in the control group. RESULTS AND CONCLUSION： The scores of forelimb placing test and beam balance test examines in the NGF, EGF or the combination group were superior to those of the control group, and the scores in the combination group were better than those in the NGF or EGF group （P 0.05）. Compared with the control group, BrdU-labelde cells were detected in the bilateral subependymal zones, the dentate gyrus of the hippocampus and damage region after traumatic brain injury in NGF, EGF or the combination treatment group increased more significantly; the expression in the combination group was better than in the NGF or EGF group （P 0.05）. Proliferation of endogenous neural stem cells and restoration of limbs function after traumatic brain injury can be promoted by NGF or EGF. Morever, this effect can be improved more significantly by the combination of NGF and EGF.

关 键 词：内源性神经干细胞 创伤性脑损伤 神经生长因子 表皮生长因子 大鼠 

分 类 号：R394.2[医药卫生—医学遗传学]