幼年大鼠脑室下区N-甲基-D-天冬氨酸受体亚单位2B的表达及其细胞类型  

Expression of N-methyl-D-aspartic acid receptor 2B in the subventricular zone of juvenile rats and its cell types

作　　者：张伟[1] 高俊英[2] 许晶[1] 崔桂云[3] 

机构地区：[1]徐州医学院2009级研究生,江苏省徐州市221002 [2]南京医科大学人体解剖学教研室,江苏省南京市210029 [3]徐州医学院附属医院神经内科,江苏省徐州市221002

出　　处：《中国组织工程研究与临床康复》2012年第1期116-120,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：江苏省自然科学基金(BK2009087);江苏省高校自然科学基金(07KJB310119)~~

摘　　要：背景:神经干细胞表达有N-甲基-D-天冬氨酸受体(N-methy-D-aspartic acid receptor,NR)亚单位2B,深入研究其在幼年大鼠脑室下区的表达情况有助于揭示该区神经干细胞增殖、分化、迁移的机制。目的:观察NR2B在幼年大鼠脑室下区的表达及表达高峰期所在的细胞类型。方法:取出生后7,14,21,28dSD大鼠,制备5μm厚脑组织冰冻切片,观察脑室下区NR2B的表达,及其在出生后14d大鼠脑室下区所在的细胞类型。结果与结论:免疫组化结果显示NR2B在出生后7,14,21,28d大鼠脑室下区存在差异表达,于出生后14d时表达达高峰,以侧脑室外侧角旁表达最高;免疫荧光双标染色可见NR2B在脑室下区与胶质纤维酸性纤维蛋白、巢蛋白、β-Ⅲ微管蛋白共定位,以室管膜细胞最明显;而与NeuN在脑室下区无共定位。说明NR2B在幼年大鼠脑室下区的表达存在时空变化,可能在神经发生中发挥作用。BACKGROUND： N-methy-D-aspartic acid receptor （NR） 2B can express on neural stem cells. In-depth study of its expression in subventricular zone （SVZ） of juvenile rats can help to reveal the mechanisms of neural stem cell proliferation, differentiation, migration. OBJECTIVE： To detect the expression of NR2B in SVZ of juvenile rats and cellular localization of NR2B on the peak of expression. METHODS： The 7, 14, 21, 28 days postnatal SD rats were used to made 5 μm frozen sections of brain tissue. The expression of NR2B in SVZ and the cellular localization of NR2B at 14 days after birth were observed. RESULTS AND CONCLUSION： Immunohistochemistry showed that there were differential expressions of NR2B in SVZ of 7, 14, 21, 28 days postnatal rats and the expression peak of NR2B was on 14 day; The expression of NR2B in the lateral angle of lateral ventricle was higher than that of other parts; NR2B was co-localized with glial fibrillary acidic protein （GFAP）, Nestin, β-Ⅲ tubulin, and ependymal cell was most obvious. However, there was no co-localization with NeuN in SVZ. The expression level of NR2B was changed following the different postnatal time. The results of double immunofluore staining implied that NR2B might play an important role in neurogenesis.

关 键 词：脑室下区 N-甲基-D-天冬氨酸受体亚单位 幼年大鼠 神经干细胞 胶质纤维酸性纤维蛋白 巢蛋白 β-Ⅲ微管蛋白 

分 类 号：R394.2[医药卫生—医学遗传学]