鼠李糖乳杆菌对Caco-2细胞抗氧化功能和细胞因子分泌的影响  

Effects of Lactobacillus rhamnosus on Anti-oxidative Functions and Cytokines Secretion in Caco-2 Cells

作　　者：黄怡[1,2] 黄琴[1] 李卫芬[1] 余东游[1] 周绪霞[1] 

机构地区：[1]浙江大学动物科学学院动物分子营养学教育部重点实验室,杭州310058 [2]广西大学动物科学技术学院,南宁530005

出　　处：《畜牧兽医学报》2012年第2期250-254,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：浙江省重大科技专项(2006C12086);国家重点基础研究发展计划(2009CB118705);博士学科点专项科研基金(20110101110101)

摘　　要：本研究旨在探明鼠李糖乳杆菌(L.rhamnosus)对Caco-2细胞抗氧化和非特异免疫功能的影响。Caco-2细胞分别用PBS(A组)、E.coli K88(B组)和L.rhamnosus(C组)处理,以及先用L.rhamnosus预处理,再用E.coli K88处理(D组)。结果表明,B组细胞上清的T-AOC浓度显著降低(P<0.01),SOD活力显著提高(P<0.01),C组与此相反,并且细胞裂解液中的SOD活性显著提高(P<0.05)。B组和C组不同程度地促进APRIL的分泌(P<0.01),而抑制IL-8的产生(P<0.01)。B组也抑制IL-10的产生,但C组却显著促进其分泌(P<0.01)。与B组相比,D组APRIL显著减少(P<0.01),但IL-8和IL-10都显著增加(P<0.05,P<0.01)。结果提示,L.rhamnosus可提高Caco-2细胞的抗氧化能力,减轻炎症反应,具有保护作用和免疫佐剂的效果。In this study, the effects of Lactobacillus rhamnosus on anti-oxidative and non-specific immunologic functions in Caco-2 cells were investigated. Caco-2 cells were treated by PBS（group A）, E. coli K88（group B） and L. rharnnosus （group C）, respectively. Caco-2 cells in group D were pre-incubated with L. rharnnosus and then infected with E. coli K88. The results showed that T-AOC concentration decreased（P〈0.01）, but SOD activity enhanced in cell culture super- natant（P〈0.01）in group B. However, the adverse situation appeared in group C and, SOD activ- ity in cell lysate increased（P〈0.05）. APRIL secretion were induced（P〈0.01）, however, IL-8 secre- tion were inhibited（P〈0.01）both in group B and group C in different degree. IL-10 production was stopped in group B, and was promoted in group C（P〈0.01）. Compared with group B, A- PRIL production significantly decreased（P〈0.01）, whereas levels of IL-8 （P〈0.05）and IL-10 （P〈0.01）increased in group D. These results suggested that Caco-2 cells were sensitize to re- spond to L. rharnnosus by an improved response in anti-oxidative function. In addition, inflam- matory response against pathogenic bacteria in enterocyte attenuated, moreover, immune re- sponse reinforced, which indicated the activities for immunoprotection and immunoadjuvant in L.rhamnosus,

关 键 词：鼠李糖乳杆菌 大肠杆菌K88 CACO-2细胞 抗氧化 细胞因子 

分 类 号：R378.992[医药卫生—病原生物学]