STAT3、CEA在人肺腺癌细胞A549中的相关性研究  

Correlation of STAT3,CEA in lung adenocarcinoma cell A549

作　　者：蓝秀[1] 王超[2] 辛彩霞[2] 宋本艳[2] 王鸿程[2] 

机构地区：[1]丽水市中心医院呼吸内科,浙江丽水323000 [2]泸州医学院附属医院呼吸内科,四川泸州646000

出　　处：《中国癌症杂志》2012年第2期130-134,共5页China Oncology

基　　金：四川省科技厅重点资助基金项目(No:2006J13-92)

摘　　要：背景与目的:肺癌的死亡率在所有癌症中居首位,其5年生存率与早期诊断密切相关。本实验通过分析信号转导和转录激活因子3(signal transducer and activator of transcription 3,STAT3)与癌胚抗原(carcinoembryonic antigen,CEA)在人肺腺癌细胞A549中的相互关系,探讨STAT3对肺腺癌早期诊断的价值。方法:采用免疫组织化学PV法、荧光定量PCR法分别检测人肺腺癌细胞A549和正常人胚肺细胞MRC-5中STAT3、CEA蛋白和mRNA表达情况,并分析在人肺腺癌细胞A549中STAT3蛋白和mRNA分别与CEA蛋白和mRNA表达的相关性。结果:STAT3、CEA蛋白和mRNA在人肺腺癌细胞A549中的表达均明显高于正常人胚肺细胞MRC-5的表达,且STAT3蛋白和mRNA的表达与CEA蛋白和mRNA的表达均呈正相关。结论:STAT3在肺腺癌的诊断中具有与CEA相同的价值。Background and purpose：Lung cancer is the leading cause of death for cancer-related deaths worldwide;its 5-year survival rate is related to an early diagnosis.The purpose of this study was to analyze the relationship between STAT3（signal transducer and activator of transcription 3） and CEA（carcinoembryonic antigen） in lung adenocarcinoma cell A549,and to explore the value of STAT3 on early diagnosis of lung adenocarcinoma.Methods：The expression of CEA,STAT3 mRNA and it＇s protein in lung adenocarcinoma cell A549 and human normal lung cell MRC-5 were tested by immunohistochemistry staining（PV） and real time PCR.The correlation between STAT3 and CEA in lung adenocarcinoma cell A549 was analyzed.Results：The protein and mRNA levels of STAT3,CEA in lung adenocarcinoma cell A549 were apparently higher than those in human normal lung cell MRC-5.The levels of STAT3 mRNA and it＇s protein were positively correlated with CEA in lung adenocarcinoma cell A549.Conclusion：STAT3 have the same value in diagnosis of lung adenocarcinoma.

关 键 词：肺腺癌 信号转导和转录激活因子3 癌胚抗原 

分 类 号：R734.2[医药卫生—肿瘤]