全自动DNA定量分析系统在宫颈癌及癌前病变筛查中的应用价值  

Value of automatic DNA image cytometry in cervical intraepithelial neoplasia and cervical cancer screening

作　　者：孟芝兰[1] 程雪梅[2] 朱晨雁 刘彤华[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院病理科,北京100730 [2]中国医学科学院北京协和医学院北京协和医院妇产科,北京100730 [3]麦克奥迪厦门病理细胞诊断中心,福建厦门361006

出　　处：《诊断病理学杂志》2012年第1期15-18,共4页Chinese Journal of Diagnostic Pathology

摘　　要：目的通过与常规细胞学诊断相比较,探讨细胞DNA定量分析系统在宫颈癌及癌前病变筛查中的应用价值。方法对2003例患者的宫颈细胞学标本分别采用常规细胞学和DNA定量分析系统检测,并将结果进行对比。结果常规细胞学检测为高级别以上的病变15例,DNA定量检测均为(+)。在细胞学检测为非典型鳞状上皮细胞(ASC-US)的57例中,28例DNA定量分析(+),25例因为非整倍体细胞未达到诊断阳性标准而建议4～6个月复查;4例为(-)。结论 DNA定量分析技术有明确的诊断标准,结果客观,可与常规细胞学结合应用于宫颈癌及宫颈癌前病变的筛查。对于细胞学检测为ASC-US的人群有分流作用,需组织学证实。Objective To investigate the value of DNA image cytometry in cervical cancer screening by comparing with the conventional cytological results.Methods There were 2003 cases involved in this study.Two thin cervical smears were made from each patient,one stained with Pap stain and another with Feulgen stain for DNA image cytometry screen.Results All of the cases diagnosed as HSIL or higher had the positive results in DNA image cytometry.57 cases diagnosed as ASCUS had different results of DNA image cytometry,among which 28 were positive,25 were recommended for re-examination within 4-6 months,and 4 were negative.Conclusion DNA image cytometry can be used to distinguish those cases of ASCUS.This computer-assisted automated method can be used in cervical cancer screening combined with conventional cytology.

关 键 词：DNA定量分析系统 宫颈癌 癌前病变 筛查 

分 类 号：R737.33[医药卫生—肿瘤]