高强度聚焦超声联合纳米微泡造影剂对兔VX2乳腺移植瘤辐照效果的影响  

High intensity focused ultrasound combined with nanoscale bubbles to damage breast VX2 tumors in rabbits

作　　者：王冬 杨珂[2] 闵加艳[1] 郭宇[1] 邹建中[1] 

机构地区：[1]重庆医科大学生物医学工程学院,省部共建超声医学工程国家重点实验室,超声医学工程重庆市市级重点实验室,重庆400016 [2]第三军医大学基础部细胞生物教研室,重庆400038

出　　处：《中国介入影像与治疗学》2012年第3期213-216,共4页Chinese Journal of Interventional Imaging and Therapy

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB707902);国家自然科学基金(60601023)

摘　　要：目的观察高强度聚焦超声(HIFU)联合纳米微泡对兔VX2乳腺移植瘤辐照效果的影响。方法制备纳米微泡,于光镜、电镜下观测微泡的大小、形态、分布及稳定性。采用Zeta SIZIER 3000电位仪测定微泡的粒径、电位。60只健康纯种雌性新西兰白兔,麻醉后种植VX2肿瘤于双侧乳腺组织内。2周后,选取乳腺区肿瘤组织直径大小为10mm的兔进行实验,随机分为实验组(HIFU+纳米微泡)和对照组(HIFU+磷酸盐缓冲液),辐照剂量150 W,辐照时间5s,记录HIFU辐照前后灰阶变化,辐照后行HE染色观察坏死区域大小,并进行统计学分析。结果成功制备纳米微泡,其理化性质稳定,粒径大小合理,电位均衡。实验组靶区平均灰度差明显高于对照组(P<0.05)。HE染色示实验组发生凝固性坏死范围明显大于对照组()。结论纳米微泡能明显增强的损伤效果。Objective To investigate the effect of high intensity focused ultrasound（HIFU） combined with nanoscale bubbles on ablation of breast tumor VX2 in rabbits.Methods Nanoscale bubbles were prepared,and the size,appearance,distribution and stability of the bubbles were observed with light microscope and electron microscope,while the diameter and Zeta potential of the bubbles were measured with Zeta SIZIER 3000 analyzer.Sixty healthy female New Zealand rabbits were injected with the tumor tissue suspension in bilateral breast tissues under anesthesia.Two weeks after injection,the rabbits with 10 mm breast tumors were selected and randomly divided into experimental group（HIFU＋nanoscale bubble） and control group（HIFU＋phosphate buffer solution）.The power of HIFU was 150 W,and the irradiation time was 5 s.The grayscale changes of the target area before and after HIFU irradiation were recorded.The coagulation necrosis after HIFU irradiation was assessed by HE staining,and statistical analysis was made.Results The nanoscale bubbles were successfully made with stable physical and chemical properties,proper diameter and equal Zeta potential.The grayscale change in the target area was significantly higher in experimental group than that in control group（P0.05）.HE staining showed the area of coagulation necrosis was significantly larger in experimental group than that in control group（P0.001）.Conclusion Nanoscale bubbles can enhance the damage effect of HIFU obviously.

关 键 词：高强度聚焦超声 纳米微泡 VX2乳腺肿瘤 兔 

分 类 号：R737.9[医药卫生—肿瘤]