大鼠下牙槽神经离断和牙周炎双重动物实验模型的建立  

Establishment of the inferior alveolar nerve transection combined experimental periodontitis animal modal in rats

作　　者：孙静[1] 李纾[1] 高艳[2] 张盼盼[3] 杨丕山[1] 

机构地区：[1]山东大学口腔医学院牙周科,山东省口腔生物医学重点实验室,山东济南250012 [2]山东省滨州医学院附属医院口腔内科,山东滨州256603 [3]山东省济南市口腔医院,山东济南250012

出　　处：《上海口腔医学》2012年第1期9-14,共6页Shanghai Journal of Stomatology

基　　金：山东省自然科学基金(ZR2010HM050)~~

摘　　要：目的:建立大鼠下牙槽神经离断和实验性牙周炎双重动物实验模型,探讨神经因素在牙周炎发生、发展及牙周组织再生中的作用,为相关牙周病学研究提供基础。方法:36只成年SPF级Wistar大鼠随机分为3组,每组12只。离断下牙槽神经并诱导牙周炎组(P1组):结扎双侧下颌第一磨牙,同时离断左侧下牙槽神经,术后高糖饮食喂养;单纯离断下牙槽神经组(P2组):离断左侧下牙槽神经,术后常规喂养;正常组(N组):假手术操作,常规喂养。采用SPSS13.0软件包对数据进行统计学处理。结果:6周时,P1组牙周炎模型建立成功,各项指标与P2组及N组差异显著;P1组离断下牙槽神经侧牙周组织破坏程度明显重于该组对照侧,P2组离断神经侧牙周组织无明显炎症。结论:神经缺失可加重牙周炎症引起的组织破坏,但单纯神经缺失不会引起牙周组织炎症。PURPOSE： To establish an animal model of the inferior alveolar nerve（IAN） transected combined experimental periodontitis in rats,in order to provide a foundation for exploring the function of nerve factor in the occurrence and development of periodontitis and periodontal tissue regeneration in vivo.METHODS： Thirty-six adult SPF Wistar rats were divided into 3 groups randomly.The IAN-transection and periodontitis group（P1）： steel-wire was used to ligate to the dental cervix of the dual first lower molars,the gingiva was lacerated using a dental probe,and the surgical transection of the IAN-transection was applied to the left side of the experimental rats,the animals were provided with high-carbonhydrate diet after surgery;the IAN-transection group（P2）： the surgical transection of the IAN-transection was applied to the left side of the experimental group,the animals were fed on in routine way after surgery;the sham surgical group（N）： no surgery was performed,the animals were fed on in routine way after surgery.6 weeks after the operation,specimens were collected by dissecting the operation areas after internal fixation and observed by clinical examination,X-ray,and histological examination.SPSS13.0 software package were used for statistical analysis.RESULTS： The IAN-transection operation was successfully conducted in the study and there was no significant necrosis or ulceration observed in the nerve dissected rats.All experimental periodontitis models were established successfully at the time point;there was severe destruction of the periodontal tissues especially in the P1 group.There was bone losses in the specimens of IAN-transection side than that in non IAN-transection side according to X-ray examination 6 weeks after operation both in the P1 and P2 group,especially in the P1 group.Periodontal tissues on both sides of the P2 group had no obvious inflammation and periodontal destruction.CONCLUSIONS： The experimental periodontitis animal model in various nerve conditions can be succ

关 键 词：下牙槽神经 失神经支配 牙周炎 大鼠 动物模型 

分 类 号：R781.4[医药卫生—口腔医学]