2种滤纸用于采集龈沟液的性能比较  

Comparison of the effectiveness of two types of filter papers on gingival crevicular fluid collection

作　　者：何艳萍[1] 谢明[1] 焦婷[1] 陈丽萍[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院.口腔医学院口腔修复科上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2012年第1期79-83,共5页Shanghai Journal of Stomatology

基　　金：上海市重点(特色)学科建设项目(T0202)~~

摘　　要：目的:比较whatman3MM层析滤纸和whatman3#定性滤纸在收集龈沟液及蛋白萃取方面的性能,为临床研究中选用合适滤纸采集龈沟液提供实验依据。方法:A组用whatman3MM层析滤纸,B组用whatman3#定性滤纸,手工裁剪成2mm×8mm大小的滤纸条。2组分别加入15个体积递增的血清品红溶液,同体积重复4次,测量每张滤纸条上的溶液浸润长度。2组分别加入5个体积递增的正常人血清,同体积重复5次,测定各组萃取液的总蛋白浓度;A、B 2组分别加入用0.9%NaCl溶液等倍稀释获得的5种浓度的正常人血清溶液1μL,同浓度重复5次,测定各组萃取液的总蛋白浓度。使用SPSS17.0软件包进行统计学分析,比较两者差异。结果:2组溶液浸润长度与溶液体积均呈现良好的线性关系,差异有显著性(P<0.05)。除体积为0.1μL时,A组滤纸条上的溶液平均浸润长度均大于B组。蛋白萃取性能比较中,2组无显著性差异(P>0.05)。结论:2种滤纸均可对龈沟液在滤纸条上的迁移距离及其所采集的龈沟液进行定量。当龈沟液分泌量较大时,whatman3#定性滤纸较3MM层析滤纸更为适用。两者的蛋白萃取性能无显著差异。PURPOSE： To compare the effectiveness of whatman3MM chromatographic filter paper and whatman3# qualitative filter paper on gingival crevicular fluid collection and protein extraction,in order to provide the evidence of using appropriate filter paper for collection of gingival crevicular fluid in clinical researches.METHODS： Whatman3MM chromatographic filter paper was used in group A and whatman3# qualitative filter paper in group B.Both types of filter papers were clipped into 2mm×8mm strips,then added into 15 incremental volumes of the fuchsine solution with serum,respectively,and each volume was repeated for 4 times.The length of infiltration on each filter paper was measured.Both groups were added into 5 incremental volumes of normal human serum separately and each volume was repeated for five times.The concentration of total protein in the extraction liquid of each group was measured.Both two groups were added into 5 incremental concentrations of 1μL normal human serum which were diluted to the corresponding multiples with 0.9% NaCl solution,and each concentration was repeated for 5 times.SPSS17.0 software package was used to conduct statistical analysis.RESULTS： The linear relation between the length of infiltration and volume of the solution were good and significant difference（P〈0.05） was found between the two groups.With the exception of 0.1μL,the lengths of infiltration in group A were all larger than that in group B.No significant difference（P〉0.05） was found in two groups when considering the protein extraction yield.CONCLUSIONS： The two filter papers were both effective to measure the volume of gingival crevicular fluid which was collected according to its infiltration length on the paper.When there was a large volume of gingival crevicular fluid,Whatman3# qualitative filter paper was more applicable than whatman3MM chromatographic filter paper.However,no significant difference was found between the two filter papers in consideration of protein extraction.

关 键 词：whatman3MM层析滤纸 whatman3#定性滤纸 龈沟液 

分 类 号：R781.4[医药卫生—口腔医学]