去唾液酸糖蛋白受体在乙型肝炎病毒感染者精子中表达的初步研究  

Preliminary study on the asialoglycoprotein receptor involving in the procedure of HBV invading human sperm

作　　者：徐婕[1] 方军[2] 靳镭[1] 彭芳昕[1] 刘世伟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院生殖医学中心,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院输血科,湖北武汉430030

出　　处：《生殖医学杂志》2012年第1期8-11,共4页Journal of Reproductive Medicine

摘　　要：目的检测乙型肝炎病毒(HBV)感染者精子中去唾液酸糖蛋白受体(ASGP R)的表达,并与HBV阴性男性精子比较,探讨ASGP-R是否参与介导HBV侵入人精子。方法用ASGP-R单抗,通过免疫印迹(Western blot)和流式细胞术检测ASGP-R在乙肝表面抗原(+)/乙肝e抗原(+)[HBsAg(+)/HBeAg(+)]者(双阳性组,n=16)、HBsAg(+)/HBeAg(一)者(单阳性组,n=28)和HBsAg(一)者(阴性组,n=35)精子中表达差异。结果 ASGP-R于人精子的胞质中高表达,于胞膜低表达;胞质中ASGP-R水平在阴性组、单阳性组及双阳性组中逐渐升高,双阳性组和单阳性组的胞膜上ASGP-R的阳性率均高于阴性组(P均<0.05)。结论人精子胞膜和胞质中均存在ASGP-R;HBV可能通过人精子表面的ASGP-R进入精子,目HBV可能通过某种机制使ASGP-R的表达量增加。Objective： To explore the mechanism of hepatitis B virus （HBV） invading into human sperm through detecting and comparing the expression of the asialoglycoprotein receptor（ASGP-R）in spermatozoa of HBV Infected male patients and the HBV-negative men. Methods： The expression levels of ASGP-R in HBV negative group（n=35）, single-positive （HBsAg -[-/HBeAg-） group （n=28）and double-positive （HBsAg-[-/HBeAg~） group （n= 16）were detected with ASGP-R monoclonal antibodies by Western blot and flow cytometry. Results： ASGP-R existed in the plasma membrane and the cytoplasm of human sperm. ASGP-R was highly expressed in human sperm membrane and lowly expressed in human sperm cytoplasm. The expres- sion levels of ASGP-R in negative group, single-positive group and double-positive group were increased gradually. There were significantly higher ASGP-R positive rates in single-positive group and double-posi- tive group than that in negative group（P^0.05）. Conclusions： There was ASGP-R expression in the plasma membrane and the cytoplasm of human sperm. HBV might invade into the human sperm by ASGP-R which exist in the plasma membrane. HBV might increase the expression levels of ASGP-R by means of some mechanism.

关 键 词：乙型肝炎病毒 精子 去唾液酸糖蛋白受体 流式细胞术 免疫印迹试验 

分 类 号：R[医药卫生]