IP6对结肠癌裸鼠皮下移植瘤细胞生长抑制及其机制的探讨  

Inhibition effect of IP6 on subcutaneous transplantation of colon cancer in nude mice

作　　者：宋扬[1] 李敏[2] 

机构地区：[1]青岛大学医学院医学营养研究所,山东青岛266021 [2]德州市疾病预防控制中心,山东德州253000

出　　处：《中华肿瘤防治杂志》2011年第23期1821-1824,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30671767);山东省自然基金(2007BS03042)

摘　　要：目的:研究肌醇六磷酸(IP6)对HT-29细胞裸鼠皮下移植瘤生长的抑制作用。方法:建立HT-29细胞裸鼠移植瘤模型,随机分为对照组(生理盐水组)、低剂量IP6组和高剂量IP6组,用药20d后处死小鼠。比较各组抑瘤作用和裸鼠一般状态的变化,透射电镜观察移植瘤细胞形体变化,TUNEL法检测移植瘤细胞凋亡情况,并运用RT-PCR、蛋白质印迹法检测移植瘤Bcl-2及Bax基因和蛋白的表达。结果:IP6组出现典型凋亡现象,PCR及蛋白检测也显示IP6可显著上调Bax蛋白的表达,下调Bcl-2蛋白的表达(P<0.05);在移植瘤质量和体积方面,用药组与对照组差异无统计意义,P>0.05,但用药组移植瘤呈现下降趋势。结论:IP6可能通过促进细胞凋亡而起到抑制和延缓肿瘤生长的作用,其机制可能与上调Bax蛋白表达和下调Bcl-2蛋白表达有关。OBJECTIVE：To investigate the effect and mechanism of inositol hexaphosphate（IP6） on human colorectal cancer in nude mouse subcutaneous xenograft model.METHODS：The cell line HT-29 in log phase of human colorectal cancer was implanted subcutaneously in BALB/C nude mice to develop implant-tumor.Then the nude mice were randomly allocated into three groups.After treating with IP6,the tumor growth and general condition of the nude mice were observed.The volume and weight of implant-tumor were measured.Simultaneously,the electron microscope and TUNEL method were used to detect and determine the cell apoptosis,and RT-PCR method was used to detect the expression of Bcl-2 and Bax mRNA in the tumor tissue.Western blot method was used to detect the expressions of Bcl-2 and Bax protein in the tumor tissue.RESULTS：IP6 induced tumor cell apoptosis and significantly inhibited the expressions of Bcl-2 and raised the expression of Bax（P〈0.05）.The volume and weight of implant-tumor in the IP6 groups were lower than those in the control group,but there was no significant difference（P〈0.05）.CONCLUSIONS：IP6 can inhibit the growth of human colorectal cancer implanted tumor mildly.The mechanism of antitumor may be correlated with inducing apoptosis by upregulating the expression of Bax and downregulating the expression of Bcl-2.

关 键 词：结肠肿瘤 基因表达 细胞凋亡 小鼠 裸 

分 类 号：R735.35[医药卫生—肿瘤]