骨髓间质细胞致瘤过程中特征基因筛查及其生物学意义的研究  

Screening of key genes of human bone marrow mesenchymal stem cell induced into tumor cell and their biology significance

作　　者：韩崇旭[1] 许文荣[2] 王文兵[3] 孙艳[4] 乌慧玲[3] 

机构地区：[1]苏北人民医院临床医学检测中心,江苏扬州225001 [2]江苏大学基础医学与医学技术学院,江苏镇江212013 [3]江苏大学生命科学研究院,江苏镇江212013 [4]苏北人民医院生殖医学中心,江苏扬州225001

出　　处：《中华肿瘤防治杂志》2011年第23期1839-1842,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：江苏省自然科学基金(BK2007072);江苏省卫生厅"科教兴卫工程"医学重点学科"实验诊断学"开放性课题(XK200723/WKF0809);江苏省苏北人民医院院内基金(yzuc-ms201039)

摘　　要：目的:研究骨髓间质细胞(MSCs)诱导致瘤的发生机制。方法:采用荧光差异显示(FDD)技术寻找MSCs诱导致瘤(F6)的关键基因。将回收的差异关键基因片段,进行PCR再扩增,TA克隆cDNA片段,测序后经BLAST软件检索以进行同源性分析。结果:FDD成功分离了MSCs和F6肿瘤细胞之间的不同基因片段,TA克隆得到15个EST片段,GenBank序列比对分析表明,这些片断均与相关基因片断同源,其中C356、G392和G383分别与nu-cleostemin,CyclinⅠ和Siva基因同源,同源百分比分别为95%、97%和100%。结论:F6肿瘤细胞的发生机制是一个涉及到众多基因参与的复杂过程,nucleostemin、CyclinⅠ和Siva等基因在人类骨髓间质干细胞诱导突变转化为F6细胞的过程中起着重要作用。OBJECTIVE：To study of the molecular mechanism of human bone marrow mesenchymal stem cell（MSCs） induced into tumor cell（F6）.METHODS：The associated key gene fragments were found to be used fluorescence label mRNA differential display technique between MSCs and F6 tumor cells were cut out from a polyacrylamide gel,re-amplified with the same primer pair.The inserts of clones were sequenced.Through BLAST software,the sequencing results were compared with GenBank database for homology analysis.RESULTS：Fifteen cDNA fragments were attained,and three of them（C356,G392 and G383） were highly up-regulated in F6 cells compared with MSCs.By comparing to the GeneBank databases,we found that C356,G392 and G383 fragments were 95%,97% and 100% homologous to nucleostemin,Cyclin Ⅰ and siva respectively.CONCLUSION：These findings support the idea that the increase of multiple genes including nucleostemin,Cyclin Ⅰ and siva expression in F6 may play a role in both tumorigenesis and transforming human bone marrow mesenchymal stem cells into F6 tumor cells.

关 键 词：骨髓细胞/细胞 间质干细胞 MRNA差异显示技术 肿瘤发生 

分 类 号：R733.3[医药卫生—肿瘤]