分光光度法测定仙鹤草总酚含量的方法研究  

Study on determining of total polyphenols content in agrimonia pilosa ledeb.by the spectrophotometry

作　　者：李倩[1] 刘子衿[1] 叶燕彬[2] 夏颖[2] 张枫[2] 

机构地区：[1]首都医科大学基础医学院 [2]首都医科大学化学生物学与药学院,北京100069

出　　处：《首都医科大学学报》2012年第1期107-110,共4页Journal of Capital Medical University

摘　　要：目的建立测定仙鹤草中总酚含量的测定方法。方法通过可见分光光度法,以没食子酸为标准品,采用Folin-Ciocalteus试剂,在装有仙鹤草样品的10mL容量瓶中,依次加入Folin-Ciocalteus试剂0.5mL,20%Na2CO31.7mL,在室温条件下放置90min,然后在波长760nm和660nm测定吸光度。结果总多酚浓度在0～10.4 mg/L与吸光度有良好的线性关系。760nm时的回归方程为y=0.091.8x+0.012.9,R2=0.999 3,加标回收率为98.4%～99.8%;660nm时的回归方程为y=0.084 7x-0.002 6,R2=0.999 7,加标回收率为101.2%～101.9%。结论在760nm和660nm 2个波长处测定的结果,都具有良好的精密度、重复性和回收率,可以作为测定仙鹤草多酚定量分析的参考方法。Objective To propose a method to determine the total polyphenols in agrimonia pilosa ledeb.Methods Folin-Ciocalteus reagent and gallic acid as the standard were used to determine the total polyphenols in agrimonia pilosa ledeb by the spectrophotometry.0.5mL of Folin-Ciocalteus reagent and 1.7mL of 20% Na2CO3 solution were added into 10mL volumetric flask which contained the certain amounts of agrimonia pilosa ledeb sample.The reaction continued of 90 min at room temperature,and then determined absorption value at 660nm and 760nm.Results The mass concentrations of polyphenols in the interval of 0~10.4 mg/L were linearly related to the absorption values.The standard curve at 760nm was y=0.091 8x＋0.012 9,R2=0.999 3 and the recovery of total polyphenols was 98.4%~99.8%.The standard curve at 660nm was y=0.084 7x-0.002 6,R2=0.999 7 and the recovery of total polyphenols was 101.2%~101.9%.Conclusion It showed good precision,repeatability and recovery to determine total polyphenols both at 760 nm and 660nm.This research provided a reference for quantitative analysis total polyphenols of agrimonia pilosa ledeb.

关 键 词：仙鹤草 分光光度法 Folin—Ciocalteus试剂 植物多酚 

分 类 号：R284[医药卫生—中药学]