固相萃取-超高效液相串联质谱法同时测定食品中红色2G、酸性大红、酸性橙Ⅱ和酸性金黄G  

Determination of Red 2G,Ponceau 2R,Orange Ⅱ and Metanil Yellow in Food by SPE-UPLC-MS/MS Method

作　　者：曾泳艇[1] 黎永乐[1] 张协光[1] 

机构地区：[1]深圳市计量质量检测研究院,广东深圳518131

出　　处：《广东化工》2012年第3期151-151,160,共2页Guangdong Chemical Industry

基　　金：质检公益性行业科研专项-食品安全预警监测与应急体系研究200910120

摘　　要：建立了食品中红色2G、酸性大红、酸性橙II和酸性金黄G非法色素的固相萃取超高效液相串联质谱测定方法。以氨水乙醇溶液作为提取溶剂,利用WAX弱阴离子交换固相萃取柱对样品进行净化。检出限红色2G0.050 mg/L,酸性大红0.050 mg/L,酸性橙Ⅱ0.010 mg/L,酸性金黄G0.010 mg/L;加标回收为78%～105%。本方法灵敏、准确高效,可对4种水油溶色素进行测定及确证。To established a method for simultaneous determination of Red 2G, Ponceau 2R, Orange Ⅱ and Metanil Yellow in food by solid-phase extraction and ultra performance liquid chromatography electrospray ionization-tandem mass spectrometry （SPE-UPLC-MS/MS） method. The samples were extracted by ethanol containing ammonia and cleaned-up by WAX solid phase extraction column. The qualitative detection limits of the method for Red 2G was 0.050 rag/L, Ponceau 2R was 0.050 mg/L. Orange 11 was 0.010 mg/L and for Metanil Yellow was 0.010 mg/L. Spike recovery of 78 % to 105 %. The method is sensitive, accurate and could be used for detecting 4 kinds of water-soluable illegal pigments in food.

关 键 词：酸性橙II 酸性金黄G 食品 固相萃取-超高效液相串联质谱 

分 类 号：O65[理学—分析化学]