Ascorbate oxidase electrochemical biosensor based on the biocompatible poly(3,4-ethylenedioxythiophene) matrices for agricultural application in crops  

作　　者：Yang Ping Wen Li Min Lu Dong Li Ming Liu Hao Hua He Jing KunXu 

机构地区：[1]Key Lab of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University,Nanchang 330045,China [2]Jiangxi Key Lab of Organic Chemistry,Jiangxi Science and Technology Normal University,Nanchang 330013,China

出　　处：《Chinese Chemical Letters》2012年第2期221-224,共4页中国化学快报（英文版）

基　　金：supported by NSFC(Nos50963002 and 51073074);Key Projects in the National Science& Technology Pillar Program in the Eleventh Five-year Plan Period of China(Nos2006BAD02A04 and 2006BAD01A01);Jiangxi Provincial Department of Education(NosGJJ11590 and GJJ10678)

摘　　要：The vitamin C（VC） in crops was successfully determined using ascorbate oxidase（AO） electrochemical biosensor based on the biocompatible poly（3,4-ethylenedioxythiophene）（PEDOT） matrices,which was easily prepared by one-step electrodeposition technique in ionic liquid microemulsions.The fabricated biosensor displayed excellent bioelectrocatalytic performance to the oxidation of VC,wide linear range,low detection limit,fast response time,good operational and storage stability,the good results of the determination of VC in vegetable crops indicated that the fabricated biosensor will be a good candidate for the physiological and biochemical studies of crops in near future.The vitamin C（VC） in crops was successfully determined using ascorbate oxidase（AO） electrochemical biosensor based on the biocompatible poly（3,4-ethylenedioxythiophene）（PEDOT） matrices,which was easily prepared by one-step electrodeposition technique in ionic liquid microemulsions.The fabricated biosensor displayed excellent bioelectrocatalytic performance to the oxidation of VC,wide linear range,low detection limit,fast response time,good operational and storage stability,the good results of the determination of VC in vegetable crops indicated that the fabricated biosensor will be a good candidate for the physiological and biochemical studies of crops in near future.

关 键 词：Biosensor Agricultural application Ionic liquid microemulsions Ascorbate oxidase Poly（3 4-ethyl-enedioxythiophene） Vitamin C 

分 类 号：O151.21[理学—数学] TP212.3[理学—基础数学]