烟碱降解微生物的筛选、鉴定及其烟碱降解效果评价  

Isolation and Characterization of Nicotine Degradation Microorganism and Evaluation of Nicotine Degradation Effect

作　　者：丁怀宇[1] 韩铭海[1] 

机构地区：[1]淮阴师范学院江苏省环洪泽湖生态农业生物技术重点实验室,江苏淮安223300

出　　处：《河南农业科学》2012年第2期82-85,共4页Journal of Henan Agricultural Sciences

摘　　要：采用烟碱降解微生物平板筛选鉴别方法,从白肋烟和烤烟中筛选出11株烟碱降解微生物,经初步鉴定,3株为细菌,8株为放线菌;采用荷移光谱法测定了11株菌对纯烟碱和白肋烟叶片中烟碱的降解能力,其中放线菌K2对烟碱的降解能力最强,对白肋烟叶片中的烟碱降解率(48h)为74.77%,对纯烟碱可以完全降解。经过生理生化试验鉴定和16SrDNA序列比对,确定放线菌K2为除虫链霉菌(Streptomyces avermitilis)。By an identification petri dish medium,11 microorganism strains which can obviously degrade nicotine were isolated from burley tobacco and flue-cured tobacco leaves.By primary identification,3 strains belonged to bacteria,and 8 strains belonged to actinomyces.Using spectrophotometric determination,the degradation ability of 11 strains on pure nicotine and burley toba-cco nicotine was examined respectively,indicating isolate K2 could almost completely degrade pure nicotine and had a maximum degradation ratio 74.77%（48 h） of burley tobacco nicotine.Based on morphological and biochemical characterization and analysis of 16S rDNA sequencing,the isolate was identified as Streptomyces avermitilis.

关 键 词：白肋烟 烤烟 烟碱 放线菌 烟碱降解 除虫链霉菌 

分 类 号：Q93[生物学—微生物学] TS411[农业科学—烟草工业]