紫外分光光度法测定鹰嘴豆豆叶、豆茎中总黄酮含量  

Determination of the Total Flavonoids in Leaves and Stems of Chickpea by UV Spectrophotometry

作　　者：阿布力米提.伊力 李桂芳 高彦华[1] 李寅庆[1] 胡尔西丹.伊麻木 刘海[3] 阿吉艾克拜尔.艾萨 

机构地区：[1]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [2]新疆塔城师范学院,新疆塔城834700 [3]新疆医科大学药学院,新疆乌鲁木齐830054

出　　处：《中国医药指南》2012年第6期1-2,共2页Guide of China Medicine

基　　金：新疆维吾尔自治区科技基础条件平台建设项目(PT0906);中国科学院"西部行动计划高新技术项目"鹰嘴豆功能因子的研究与开发(KGCX2-YW-503);973计划前期研究专项(2009CB526509)

摘　　要：目的建立鹰嘴豆豆叶,豆茎中总黄酮含量的测定方法。方法采用紫外分光光度法测定药材中总黄酮含量。结果采用紫外分光光度法测定总黄酮含量时,在0.003~0.018mg/mL浓度范围内线性关系良好（r=0.9998）,豆叶和豆茎的平均回收率为99.3%和101.1%,RSD为1.86%和2.46%。结论所用方法简便、准确,可用于鹰嘴豆的质量控制。Objective To study the determination of the total flavonoids from leaves and stems of chickpea. Method Determine the total flavonoids by ultraviolet spectrophotometry. Results There is a good linear relationship（r=0.9998） in the concentration range between 0.003 to 0.018mg/mL. The average recovery rate of the total flavonoids from bean leaves and stems is 99.3% and 101.1%, and RSD is 1.86% and 2.46%. Conclusion The method is simple, accurate and can be used for the quality control of chickpea.

关 键 词：鹰嘴豆 总黄酮 紫外分光光度法 

分 类 号：R282.710[医药卫生—中药学]