抗CD3抗CEA抗体诱导杀伤细胞对胃癌体内杀伤的作用  

The effect on gastric cancer of CIK cell mediated by bispecific single-chain antibody in vivo

作　　者：刘洪一[1] 李荣[2] 刘巨超[2] 李力[2] 梁文涛[2] 钟苑[2] 徐迎新[2] 

机构地区：[1]解放军总医院肿瘤外科,北京100583 [2]解放军总医院普通外科研究所,北京100853

出　　处：《中国肿瘤临床与康复》2012年第1期5-7,共3页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：国家高技术研究发展计划(2006AA02Z4B9)

摘　　要：目的探讨抗CD3抗CEA双特异性单链抗体诱导杀伤细胞(CIK细胞)在体内杀伤胃癌的作用。方法将BGC823细胞种植裸鼠后,建立裸鼠胃癌模型,分为3组,分别经尾静脉注入生理盐水、CIK细胞和CIK细胞+双特异性单抗,每3天治疗1次,共6次,取出肿瘤组织称重,并计算出各组的抑瘤率。结果在体内,CIK组和CIK+双特异性单抗组均可抑制肿瘤生长,抑瘤率分别为27.4%和41.7%,与对照组比较,差异均有统计学意义(均P<0.05)。CIK组与CIK+双特异性单抗组的瘤体积、瘤重、抑瘤率比较,差异均有统计学意义(均P<0.05)。结论 CIK细胞本身可以抑制肿瘤细胞的生长,在加入抗CD3抗CEA双特异性单链抗体后,抑瘤作用明显增强。Objective To investigate the killing effect on gastric cancer cells of CIK cells mediated by bispecific single-chain antibody in vivo. Methods To establish gastric cancer model in nude mice with BGC823 cells. And the mice are divided into 3 groups, which are injected with normal saline, CIK ceils and CIK cells ＋ bispecific antibody, respectively, once every 3 days for 6 times. Then remove and weigh the tumors, and calculate the inhibition rate in each group. Results The CIK cells and CIK cells with bispecif- ic single-chain antibody can both inhibit tumor growth in vivo. To compare with the control group, the inhibi- tion rates were 27.4% and 41.7% respectively （P 〈0. 05）. To compare with CIK cells, CIK cells with bis- pecific single-chain antibody can inhibit tumor growth （P 〈 0. 05）. Conclusion CIK cells alone can inhibit tumor cell growth, associated with the anti-CEA anti-CD3 bispecific single-chain antibody can significantly enhance antitumor effect.

关 键 词：胃肿瘤 杀伤细胞 双特异性单链抗体 

分 类 号：R735.2[医药卫生—肿瘤] R73-354[医药卫生—临床医学]