芥菜cry2基因RNAi载体的构建与遗传转化研究  

Construction of RNAi Vector of Cryptochrome 2 Gene and the Transformation of Brassica juncea

作　　者：梁婷[1,2] 刘毅[1,2] 蔡应繁[1] 孙全[1] 朱小燕[1] 岳显可[1,2] 江怀仲[1] 何晓红[1] 

机构地区：[1]重庆邮电大学生物信息学院,重庆400065 [2]成都中医药大学研究生院,四川成都611130

出　　处：《安徽农业科学》2012年第7期3894-3898,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(31071461;30771311);重庆市重点攻关项目资助(CSTC2011AB1095);重庆市教委项目(KJ110506);重庆市自然科学基金(CSTC2009BA1088;cstc2007BB1328)

摘　　要：[目的]研究芥菜Cry2基因RNAi载体的构建与遗传转化,为后期对两者关系的进一步研究提供参考。[方法]在克隆芥菜类茎瘤芥Cry2基因的基础上,构建该基因的RNAi载体,并导入根瘤农杆菌GV3103,获得工程菌株后,通过农杆菌浸染茎瘤芥子叶外植体进行植物体转化,并验证转化结果。[结果]成功构建了茎瘤芥Cry2基因的RNAi载体,并建立了茎瘤芥遗传转化体系,获得了转基因植株。[结论]茎瘤芥Cry2 RNAi的构建及遗传转化体系的建立为后期深入研究茎瘤芥熟期相关基因的功能及基因工程应用奠定了基础。[Objective]Cryptochrome gene is one of the key genes in the light signal pathway which have important impacts on plant flowering.The Cryptochrome control seedling de-etiolation,dependent inhibition of Hypocotyl elongation,control of nutrition and anthocyanin accumulation,and the maintenance of plant endogenous rhythms in plants.research mustard cry2 gene RNAi carrier construction and genetic transformation Based on this study.[Method]The research constructed the RNAi vector of cryptochrome 2 gene based on cloning of the gene from Tumorous stem mustard.[Result]Then we successfully transfered it into agrobacterium tumefaciens strain GV3101 to form engineering strain.Dipped into plant callus through Agrobactrium tumefaciens,acquire transgenic plants.[Conclusion]The construction of cry2 RNAi and the establishing of genetic transformation system set fundation for the research of the funtion of related gene in Tumorous stem mustard and the appllication of genetic engineering.

关 键 词：茎瘤芥 隐花色素2 RNA干涉 遗传转化 

分 类 号：S637[农业科学—蔬菜学] S603[农业科学—园艺学]