养殖丁肝胰脏淀粉酶活性的初步研究  

Study on Amylase Activities of the Hepatopancreas in Cultured Tinca tinca L.

作　　者：马诚[1] 朱敏[1] 李英文[1] 

机构地区：[1]重庆市高校动物生物学重点实验室重庆市高校生物活性物质工程研究中心重庆师范大学生命科学学院,重庆400047

出　　处：《安徽农业科学》2012年第7期4013-4016,4140,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究养殖丁(Tinca tinca L.)肝胰脏淀粉酶的基本性质。[方法]采用酶学分析方法研究丁肝胰脏淀粉酶的pH、温度、底物浓度、缓冲液及8种金属离子对其肝胰脏淀粉酶活性影响。[结果]淀粉酶最适pH为7.5,最适温度44℃,最适底物浓度0.4%,最适缓冲液为0.2 mol/L Tris-HCl缓冲液。在设定浓度范围内(10～50 mmol/L),K+、Na+对淀粉酶活力影响具有促进作用;Ca2+、Mg2+对淀粉酶活力也有促进作用;Fe3+在低浓度(10 mmol/L)表现为促进,高浓度(20～50 mmol/L)表现为抑制作用;Al3+、Pb2+、Ag+对酶活力具有抑制作用。[结论]结果为丁鱼岁的人工养殖和饲料优化,提供一定的理论依据。[Objective] The study aimed to study the basic properties of amylase of hepatopancreas in cultured T.tinca.[Method] Using enzyme analysis method,the effects of optimal pH,temperature,substrate concentration,buffer and 8 kinds of metal ions on amylase activities were studied.[Result] The optimal pH is 7.5,the temperature is 44 ℃,the substrate concentration is 0.4% and the optimal buffer is 0.2 mol/L Tris-HCl buffer.Within the gradient concentrations（10 to 50 mmol/L）,K＋ and Na＋ have promoting action on amylase activities;Ca2＋ and Mg2＋ also have promoting effect;while Fe3＋ has promoting effect at low concentration（10 mmol/L）,but inhibit amylase activities at high concentration（20 to 50 mmol/L）,and Al3＋,Pb2＋ and Ag＋ could inhibit amylase activities.[Conclusion] The results provide certain theoretical basis for artificial culture and feed optimization of T.tinca.

关 键 词：丁 淀粉酶活力 金属离子 

分 类 号：S963[农业科学—水产养殖]