白藜芦醇对肝癌细胞增殖和凋亡影响及其机制  

Effects of Resveratrol on Cell Cycle and Apoptosis of Hepatocellular Carcinoma Cells and its Possible Mechanism

作　　者：戴维奇[1] 徐凌[1] 王锋[1] 卫巍[1] 沈杰[1] 黄银实[1] 杨丽娟[1] 何姗姗[1] 郭传勇[1] 

机构地区：[1]同济大学附属第十人民医院消化内科,上海200072

出　　处：《现代生物医学进展》2012年第1期16-19,26,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金(81072005);上海市科委基金(09410705400)

摘　　要：目的:研究白藜芦醇(resveratrol,Res)对肝癌细胞LM3周期及凋亡的影响并探讨其可能的分子机制。方法:分别用50、100、150、200μmol/L浓度的Res作用LM3肝癌细胞24、48和72h,CCK-8测定Res对细胞的增殖作用;分别以相同剂量的DMSO及无处理的LM3细胞为随机和空白对照,观察150μmol/L Res作用72小时后对肿瘤细胞周期及凋亡的影响;Real time-PCR及Western blot分析Res对LM3细胞中Bak和Bcl-2 mRNA及蛋白表达的影响。结果:Res体外能明显抑制肝癌细胞LM3的生长增殖,在一定范围内呈现作用浓度(F=101.183,P<0.001)和时间依赖性(F=192.371,P<0.001);150μmol/L Res作用72小时后能显著减缓肝癌细胞LM3的周期转换(P<0.001),并诱导明显的细胞凋亡效应(P<0.001);进一步的检测发现Res作用LM3细胞后,Bak mRNA(P=0.002,0.007)及蛋白(P=0.004,0.01)表达增强,而Bcl-2 mRNA(P=0.027,0.007)及蛋白(P=0.001,0.001)表达出现显著下降。结论:Res可能通过对Bak及Bcl-2表达的调节来抑制肝癌细胞LM3的增殖,并诱导其细胞凋亡。Objective： To investigate the effect of resveratrol（Res） on cell cycle and apoptosis of hepatocellular carcinoma LM3 cells and the possible molecular mechanisms.Methods： Cell counting kit-8（CCK-8） assay was used to examine the effect on cell prolifer-ation of hepatocellular carcinoma LM3 cells treated by Res with the concentration of 50μmol/L for 24 and 72 hours;LM3 cells were treated by DMSO or no treatment as randomized and blank control,and then were detected.The expression of Bak and Bcl-2 mRNA and protein in LM3 cells treated by Res were evaluated by Real-time PCR and western blot,respectively.Results： Res inhibited the prolifera-tion of LM3 cells in time-dependent manner（F=101.183,P0.001） and concentration-dependent manner（F=192.371,P0.001）in vit-ro.It was found that Res could accelerate cells cycle transition and induce apoptosis after treatment by Res at 150μmol/L for 72 hours.The expression of Bak mRNA（P=0.002,0.007） and protein（P = 0.004,0.01）were obviously up-regulated on treated LM3 cells,while the expression of Bcl-2 mRNA（P=0.027,0.007） and protein（P = 0.001,0.001）decreased remarkably.Conclusion： Res can not only in-hibit proliferation of LM3 cells but also induce cellular apoptosis through accommodating the expression of Bak and Bcl-2.

关 键 词：肿瘤 周期 BCL-2 BAK 

分 类 号：R735.7[医药卫生—肿瘤] R285.6[医药卫生—临床医学]