巨噬细胞对卵巢癌细胞SKOV3生物学功能的影响  

Influence of tumor associated macrophages on biological function of SKOV3 cell

作　　者：朱亚飞[1] 高国兰 杨小荣[1] 

机构地区：[1]南昌大学第一附属医院妇产科,南昌330006 [2]北京航空总医院妇产科,北京100012

出　　处：《现代妇产科进展》2012年第2期94-97,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:研究巨噬细胞对卵巢癌细胞株SKOV3生物学功能的影响。方法:(1)体外采用IL-4和佛波醇酯(PMA)分别诱导M2和M1型巨噬细胞,流式细胞仪鉴定分型;(2)Tranwell小室建立巨噬细胞与卵巢癌细胞SKOV3体外非接触式共培养模型。比较共培养后,SKOV3的增殖和凋亡、迁移和侵袭的变化。MTT法检测增殖;流式细胞仪Annexin V-FITC/PI双染检测凋亡;Transwell检测侵袭和迁移。结果:(1)IL-4诱导的巨噬细胞高表达CD163,为M2型,PMA诱导组高表达HLA-DR,为M1型。SKOV3和普通巨噬细胞共培养后,巨噬细胞CD163高表达。(2)SKOV3的增殖和凋亡:M2共培养组SK-OV3的增殖活性显著高于M1共培养组和普通共培养组(P<0.05)。M2共培养组SKOV3的凋亡率显著低于M1共培养组和普通共培养组(P<0.05)。(3)SKOV3的迁移和侵袭:M2共培养组SKOV3的侵袭能力显著强于M1共培养组和普通共培养组(P<0.01)。M2共培养组SKOV3的迁移能力显著强于M1共培养组和普通共培养组(P<0.05)。结论:共培养卵巢癌细胞使巨噬细胞M2表型极化。M2型巨噬细胞促进卵巢癌细胞增殖,提高侵袭、迁移能力,减少凋亡,而M1型起相反作用。Objective：To investigate the influence of tumor-associated macrophages （TAMs＇） on the biological function of human ovarian cell line SKOV3. Methods：Macrophage was induced into M2 subtype macrophage form with intedeukin（IL）-4 plus PMA, M1 subtype with LPS plus PMA respectively, macrophage scarenger receptor（ CD163 ） were analyzed with flow cytometry. SKOV3 was co-cultured with TAMs in the transwell. Apoptosis and proliferation of SKOV3 were detected with MTT and Annexin V-PI flow cytometry,, migration and invasion capability were measured by transwell assay respectively. Results： Cocuhuring with SKOV3 in- duced M2 subtype macrophage with elevated expression of CD163. After co-cultured with M2 macrophage, the activity of apoptotic rates decreased and the proliferation, the migration and invasion capability increased for cocultured SKOV3. Conclusion： Cocuhured with SKOV3, macrophages tended to polarized into a M2 subtype, M2 macrophage may contribute to cancer progression by inhibiting the apoptosis while promoting the proliferation, migration and invasion capability of SKOV3. while M1 macrophage plays an opposing effect.

关 键 词：卵巢肿瘤 巨噬细胞 体外 

分 类 号：R737.31[医药卫生—肿瘤]