甘遂对水负荷小鼠排尿以及肾脏AQP2,IL-1β,TNF-α mRNA表达的影响  

Effect of Euphorbia kansui on urination and kidney AQP2,IL-1β and TNF-α mRNA expression of mice injected with normal saline

作　　者：李慧玉[1] 雷帆[1] 王玉刚[1] 肖新月[2] 胡珺[1] 程显隆[2] 邢东明[1] 花雷[1] 林瑞超[2] 杜力军[1] 

机构地区：[1]清华大学生命科学学院,医学院药物药理研究室,蛋白质科学教育部重点实验室,北京100084 [2]中国食品药品检定研究院,北京100050

出　　处：《中国中药杂志》2012年第5期606-610,共5页China Journal of Chinese Materia Medica

基　　金：国家“十一五”科技支撑计划项目(2006BAI14B01);国家自然科学基金项目(81073092,30973896);国家“重大新药创制”科技重大专项(2011ZX09101-002-11,2012ZX09103-201-041,2012ZX09102-201-008)

摘　　要：目的:观察甘遂醇提取物对水负荷小鼠利尿排水以及肾脏相关因子表达的作用,以探讨其对肾脏的影响。方法:利用腹腔注射生理盐水负荷小鼠模型和正常小鼠,分别观察甘遂灌胃给药后利尿以及肾脏相关组织细胞因子的变化。结果:甘遂醇提取物对水负荷小鼠具有促进利尿的作用。同时升高外周血清肌酐,组织切片观察未见到明显的病理变化。对下调肾集合管AQP2表达有一定的作用趋势,并能够促进表达TNF-α。结论:甘遂大剂量对肾脏具有确实的作用,并可能伴随有一定的病理反应,其中以1.2 g.kg-1剂量明显。这种病理反应与血清肌酐升高和TNF-α表达变化相关。Objective： To observe the effect of Euphorbia kansui（E.KS） alcohol extracts on urination and kidney-related expressions of mice injected with normal saline and to discuss its impact on kidney.Method： Mice intraperitoneally injected with normal saline were observed for urination and changes in kidney-related histiocytic factors of after intragastrical administration of E.KS and compared with normal mice.Result： E.KS alcohol extracts can promote urination of mice injected with normal saline and enhance peripheral serum creatinine,with no obvious pathological change showed in tissue sections.It had a certain effect on reducing AQP2 expression and enhancing TNF-α expression.Conclusion： Euphorbia kansui in large dose has a remarkable effect on kidney but may be accompanied with pathological reactions to some extent,especially the dose of 1.2 g·kg-1.The pathological reactions may be related with increased serum creatinine and TNF-α expression.

关 键 词：甘遂 肾脏 AQP2 TNF-Α 肌酐 

分 类 号：R285.5[医药卫生—中药学]