人乳腺癌组织的特征红外光谱研究  

Distinctive Infrared Spectral Features in Human Breast Cancer

作　　者：沈世杰[1] 刘炳玉[1] 李清[2] 马溪[1] 宋占军[1] 

机构地区：[1]军事医学科学院国家生物医学分析中心,北京100850 [2]军事医学科学院307医院病理科,北京100850

出　　处：《光谱学与光谱分析》2000年第1期28-30,共3页Spectroscopy and Spectral Analysis

基　　金：国家科技部条财司 99年度分析测试专题资助项目

摘　　要：应用傅里叶变换红外光谱对 2 0例人乳腺病理样品进行了分析。实验发现 ,乳腺癌组织与乳腺良性病变组织之间有较大的红外光谱差异。与乳腺良性病变组织比较 ,乳腺癌组织的红外光谱中磷酸二酯基团反对称伸缩振动向短波方向位移了 2cm-1,其对称伸缩振动向短波方向位移了 3cm-1;CO伸缩振动吸收A1173/A116 3比值增加 ,A10 2 5/A10 82 比值下降 ;CH3的反对称及对称伸缩振动减弱 ,CH2 的对称及反对称伸缩振动增强。Substantial differences were found in the spectral properties of surgical samples from 20 women patients with histologically normal and cancerous breast tissue.The most striking changes in the spectra were observed in the symmetric and asymmetric stretching bands of phosphodiester groups,which shifted to short wavenumber about 3 cm -1 in ν sPO - 2,and to long wavenumber about 2 cm -1 in ν sPO - 2 .The ratio of A 1 173 /A 1 163  increased and that of A 1 025 /A 1 082  decreased,the intensity of symmetric and asymmetric stretching bands of CH 3 decreased and those of CH 2 increased in all of breast cancer tissues.Our findings indicated that in breast cancer tissue,the degree of hydrogen bonding of oxygen atoms in the backbone of nucleic acid increased;the content of glycogen decreased;the degree of hydrogen bonding of OH groups in serine,tyrosine,and threonine residues of cell proteins decreased;and also there were changes in the packing and the conformational structure of the methylene chains of membrane lipids.

关 键 词：乳腺癌 红外光谱 人 癌组织 癌变过程 

分 类 号：R737.904[医药卫生—肿瘤] R445.7[医药卫生—临床医学]