鹰嘴豆肽抑制肿瘤作用和对免疫功能的影响  

Antitumor and Immune-Enhancing Activity of Chickpea Peptides in Hepatoma H22-Bearing Mice

作　　者：高捷[1] 王华[1] 寇晓虹[1] 薛照辉[1] 

机构地区：[1]天津大学化工学院食品科学系,天津300072

出　　处：《食品科学》2012年第3期215-219,共5页Food Science

基　　金：天津大学创新基金项目(2010XJ-0216)

摘　　要：目的:探讨鹰嘴豆肽抑制肝癌H22细胞移植瘤的效果及对免疫功能的影响。方法:将小鼠随机分为正常对照组、阳性对照组、肿瘤对照组和鹰嘴豆肽低、中、高剂量组(50、100、200mg/(kg·d)),除正常组外,其余各组小鼠均在皮下接种肝癌H22细胞,建立肝癌小鼠模型。建模后第2天开始灌胃给药,连续给药10d。末次给药24h后拉托颈椎处死小鼠,测体质量、肿瘤大小、抑瘤率、胸腺指数、脾指数、巨噬细胞吞噬能力、脾细胞吞噬能力等。结果:鹰嘴豆肽低、中、高3个剂量均可抑制H22肿瘤的生长,且没有阻碍小鼠体质量和免疫器官的增长。与肿瘤对照组相比,鹰嘴豆高剂量组可以显著提高小鼠迟发型超敏反应(DTH)的能力、淋巴细胞和巨噬细胞吞噬能力(P<0.05),鹰嘴豆低、中剂量组对其也有一定的改善作用,但是没有表现出剂量依赖效应。讨论:鹰嘴豆肽可能是通过增强H22肝癌小鼠的免疫功能来抑制肿瘤的生长,具体的作用机制还需要更深入的研究。Objective： To find out whether chickpea peptides has antitumor and immune-enhancing activity in hepatoma H22-bearing mice.Methods： KM mice were randomly divided to normal control group,positive control group（PC）,tumor control group and chickpea peptide groups at the doses of 50,100 mg/（kg·d） and 200 mg/（kg·d）.The H22-bearing mice were treated with chickpea peptide and Cy by gavage at the volume of 0.2 mL/mouse per day for 10 consecutive days.Distilled water was used for the normal control group.The body weight,tumor growth,tumor inhibitory rate,thymus index,spleen index,cell-mediated immune functions including lymphocyte proliferation,peritoneal macrophage phagocytosis and splenic lymphocytes were detected at 24 h after the last administration.Results： An inhibitory tend of tumor growth was observed in the chickpea peptide group.Compared with the tumor control group,a significant increase in relative spleen weight,delayed-type hypersensitivity（DTH）,peritoneal macrophage phagocytotic activity and splenic lymphocyte in the chickpea groups was also observed.However,no dose-dependent effect was achieved.Conclusion： Peptides derived from chickpea have anti-tumor activity probably by enhancing immune functions in hepatoma H22-bearing mice.The detailed mechanism remains to be further studied.

关 键 词：鹰嘴豆肽 抗肿瘤 H22肿瘤 免疫功能 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]