TIP30基因启动子甲基化与大肠癌细胞奥沙利铂化疗敏感性关系的研究  

Study on correlation of the methylation status of CpG islands in the promoter region of TIP30 tumor suppressor gene with the L-OHP chemosensitivity in 2 colorectal cancer cells

作　　者：陈小兵[1,2] 马一杰[1,2] 陈贝贝[1,2] 吕慧芳[1,2] 李宁[1,2] 邓文英[1,2] 罗素霞[1,2] 

机构地区：[1]郑州大学附属肿瘤医院 [2]河南省肿瘤医院内科,河南郑州450008

出　　处：《中国医药科学》2012年第4期14-16,共3页China Medicine And Pharmacy

基　　金：河南省医药卫生重大攻关项目;卫生部科研基金资助项目(WKJ2011010012)

摘　　要：目的探讨TIP30启动子CpG岛甲基化状态与大肠癌细胞对奥沙利铂敏感性的关系。方法采用MTT法检测HCT116和HT29大肠癌细胞株对L-OHP的敏感性。通过甲基化特异性PCR,检测对奥沙利铂敏感性有差异的2个大肠癌细胞株中TIP30基因启动子CpG岛甲基化状态,并用RT-PCR法检测其mRNA表达。结果 HCT116及HT29细胞中TIP30基因甲基化状态存在差异,其表达水平与启动子CpG岛甲基化状态有关,并且二者对奥沙利铂的敏感性不同。结论 TIP30基因启动子甲基化状态可能影响大肠癌细胞对化疗药物的敏感性,为大肠癌患者的个体化治疗提供了依据。Objective To explore the relationship between the methylation status of CpG islands in the promoter region of TIP30 genes in colorectal cancer cells and their sensitivity to Oxaliplatin in the 2 colorectal cancer cells.Methods MTT assay was applied to evaluate the sensitivity to 5-Fu in HCT116 and HT29 colorectal cells;Methylation-specific PCR was used to detect promoter CpG island methylation status in HCT116 and HT29colorectal cells;RT-PCR was used to measure TIP30 mRNA expression.Results TIP30 gene displayed differential DNA methylation pattern between the HCT116 and HT29 cell lines.The mRNA expression level of it was correlated with the methylation status of CpG islands.The sensitivity to L-OHP was different in HCT116 and HT29 cells.Conclusion Our data indicate that methylation status of TIP30 is associated with chemosensitivity in colorectal cells.It may make individualized treatment be possible in patients with colorectal cancer.

关 键 词：大肠癌 DNA甲基化 奥沙利铂 化疗药物敏感性 

分 类 号：R735.34[医药卫生—肿瘤]