斑贞1号、川芎嗪联合5-氟尿嘧啶对人胃癌细胞谷胱甘肽S-转移酶的影响  

Banzhen 1,Tetra methylpyrazine and 5-fluorouracil effect on human gastric cancer cells Glutathione-S-transferase

作　　者：付桂珍[1] 张爱民[2] 

机构地区：[1]内蒙古自治区化德县医院内科,内蒙古化德013350 [2]包头医学院第一附属医院消化科,内蒙古包头014010

出　　处：《中国医药科学》2012年第4期34-35,共2页China Medicine And Pharmacy

摘　　要：目的探讨斑贞1号、川芎嗪和5-氟尿嘧啶联合逆转人胃癌细胞系SGC-7901/ADR与谷胱甘肽S-转移酶表达的相关性。方法采用半定量逆转录聚合酶链反应比较人胃癌细胞SGC-7901/ADR细胞在单纯5-FU和5-FU联合斑贞1号及TMP处理组GST-πmRNA的表达情况。结果 5-FU组与阴性对照组相比,GST-πmRNA表达差异无统计学意义(P>0.05),而5-FU联合斑贞1号及TMP处理组GST-πmRNA表达量明显降低(P<0.05)。结论斑贞1号、TMP和5-FU联合逆转SGC-7901/ADR细胞耐药性可能与GST-π相关,为当前胃癌的治疗提供新的理论依据。Objective To explore of the correlativity between Banzhen 1,Tetra methylpyrazine and 5-fluorouracil reversing human gastric cancer cell line SGC-7901/ADR with Glutathione-s-transferase expressions.Methods Semiquantitative reverse transcriptase polymerase chain reaction was used for comparison of GST-πmRNA expression for cell line SGC-7901/ADR in group of 5-FU,5-FU and Banzhen 1,TMP.Results The expression of GST-πmRNA between the 5-FU group and the control group,there was no significant differences （P 0.05）,while 5-Fu and Banzhen 1 group and TMP group was decreased significantly（P0.05）.Conclusion It is possible correlated to GST-π for Banzhen 1,TMP and 5-FU reversed multidrug resistance in human gastric cancer cells,which may supply a new theoretical basis for the clinical effective treatment of gastric carcinoma.

关 键 词：胃癌 斑贞1号 TMP 5-FU GST-Π 

分 类 号：R735.2[医药卫生—肿瘤]