亚洲栽培稻程氏指数与SSR标记分类的比较分析  

Comparison of Cheng's Index-and SSR Markers-based Classification of Asian Cultivated Rice

作　　者：王彩红[1] 徐群[1] 于萍[1] 袁筱萍[1] 余汉勇[1] 王一平[1] 汤圣祥[1] 魏兴华[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006

出　　处：《中国水稻科学》2012年第2期165-172,共8页Chinese Journal of Rice Science

基　　金：农业部物种资源保护专项基金资助项目

摘　　要：选取具有代表性的100份亚洲栽培稻品种,应用程氏指数和109个SSR分子标记对它们进行分类。结果表明,等位酶Ⅰ群(典型籼稻)和Ⅵ-A群(温带粳稻)程氏指数和SSR标记亚结构特征均十分明显,拟合度较高(73.3%～100%)。Mantel检测揭示,程氏指数与SSR分子标记进行栽培稻分类存在明显的正相关,两种方法各个材料间遗传距离的相关达极显著水平(r=0.466,P<0.01)。遗传结构和聚类分析表明,在籼粳亚种水平上,程氏指数和SSR标记分类结果趋于一致,但在亚种内亚结构分析中,程氏指数偏差较大,SSR标记具有更高的准确性。同时,SSR分析表明混合型材料多存在于Ⅱ群和Ⅵ群内部。A total of one hundred cultivated rice accessions were grouped based on Cheng＇s index and SSR polymor- phism. The results showed that the subpopulation features of allozyme group I （indica） and allozyme group VI-A （temperate japonica） were obvious both classified by Chengs index and SSR marker, with goodness of fit ranging from 73.3% to 100%. Mantel test revealed that the Euclidean distance of Cheng＇s index was significantly correlated with Nei＇s genetic distance of 109 SSR loci （r=0. 466, P（0.01）. According to both model-based structure and UPGMA cluster analysis, the classification results at subspecies level based on Cheng＇ s index coincided with that on SSR marker. Within subspecies, the subpopulations classified by Cheng＇s phenotypic characters showed lower goodness of fit than SSR marker. In addition, allozyme group H （aus, boro） and allozyme group VI （temperate japonica, tropical japonica） had more mixture types based on SSR locus analysis.

关 键 词：亚洲栽培稻 分类 程氏指数 简单重复序列 

分 类 号：S511[农业科学—作物学]