靛玉红对喉癌Hep-2细胞侵袭能力的影响及机制  

Effects and Mechanism of IRO on Hep-2 cells invasion

作　　者：周红[1] 张军辉[2] 

机构地区：[1]河南省平顶山市第一人民医院耳鼻喉科,467000 [2]郑州大学第三附属医院耳鼻咽喉科,450052

出　　处：《中国医疗前沿》2012年第2期12-13,2,共3页China Healthcare Innovation

摘　　要：目的探讨靛玉红甲肟(Indirubin-3'-monoxime,IRO)对人喉癌Hep-2细胞迁移和侵袭的影响及其机制。方法MTT法检测不同浓度、不同作用时间IRO对Hep-2细胞增殖活性的影响。Boyden chamber实验计算各组穿膜的细胞数以比较靛玉红甲肟对细胞侵袭能力的变化。明胶酶谱检测不同浓度靛玉红甲肟处理喉癌Hep-2细胞后细胞MMP-9和MMP-2活性的变化。结果 MTT结果发现IRO对Hep-2细胞具有明显的增殖抑制作用,且表现为剂量依赖性(P<0.01)。Boydenchamber体外迁移和侵袭实验结果显示:靛玉红甲肟可以显著抑制人喉癌Hep-2细胞的侵袭能力(P<0.05)。明胶酶谱检测发现以靛玉红甲肟处理24h后人喉癌Hep-2细胞MMP-9和MMP-2活性明显降低,不同浓度组间存在显著性差异。结论靛玉红甲肟具有抑制人喉癌Hep-2细胞株增殖和侵袭的作用,其作用机制与显著抑制MMP-9和MMP-2活性有关。Objective To study the effect of Indirubin-3＇-monoxime（IRO） on human laryngeal carcinoma Hep-2 cells invasion and to explore its mechanism.Methods Methabenzthiazuron（MTT） was used to observe the proliferation of Hep-2 cells treated with various concentrations of IRO at different time.Boyden chamber was used to count each group worn membranous cell count and to compare IRO effect on cell invasion.Gelatin Zymography was used to the activity of MMP-9 and MMP-2.Results IRO inhibited growth of Hep-2 cells in a dose-dependent and time-dependent manner（P〈0.01）.IRO can significantly inhibit human laryngeal cancer Hep-2 cell invasion（P〈0.05）.The activity of MMP-9 and MMP-2 decreased in a dose dependent manner after the treatment by IRO.Conclusions IRO inhibit human laryngeal cancer Hep-2 cell proliferation and invasion,its mechanism is related to down-regulate MMP-9 and MMP-2 activity.

关 键 词：喉癌 靛玉红甲肟 HEP-2 MMP-9 MMP-2 

分 类 号：R739.6[医药卫生—肿瘤]