浸润性乳腺癌SFRP1基因的表达及其启动子甲基化的意义  

The significance of mRNA expression and methylation of SFRP1 promoter in invasive breast cancer

作　　者：齐凤杰[1] 张丽[1] 赵树鹏[1] 

机构地区：[1]辽宁医学院病理教研室,辽宁锦州121001

出　　处：《广东医学》2012年第4期469-472,共4页Guangdong Medical Journal

基　　金：辽宁省科学技术计划项目(编号:2010225034)

摘　　要：目的探讨浸润性乳腺癌及相应癌旁乳腺组织中分泌型卷曲相关蛋白1(the secreted frizzled-relat-ed protein 1,SFRP1)基因的表达及其启动子CpG岛的异常甲基化状态,并分析其临床意义。方法采用实时荧光定量PCR技术及甲基化聚合酶链反应(MSP)技术检测58例浸润性乳腺癌组织和相应癌旁乳腺组织中SFRP1基因的mRNA表达及其启动子CpG岛的异常甲基化情况,并分析其与临床病理参数之间的关系。结果浸润性乳腺癌组SFRP1基因的甲基化显著高于癌旁乳腺组织组(P<0.05);腋窝淋巴结有转移的浸润性乳腺癌组SFRP1基因的甲基化显著高于腋窝淋巴结无转移组(P<0.05),而与其他临床病理参数无关。SFRP1在浸润性乳腺癌组的mRNA表达量与乳腺癌旁组比较,差异有统计学意义(P<0.05);在23例SFRP1基因启动子CpG岛发生异常甲基化的浸润性乳腺癌组,均未检测到SFRP1的mRNA表达。结论 SFRP1基因异常甲基化可能影响其mRNA的转录水平;SFRP1基因启动子的甲基化与浸润性乳腺癌的发生发展有关,对其进行检测有可能为浸润性乳腺癌的早期诊断和判断预后提供帮助。Objective To investigate the mRNA expression and methylation of SFRP1 promoter in invasive breast cancer and adjacent normal breast tissues,and to evaluate the clinical significance.Methods The mRNA expression and methylation of SFRP1 gene in 58 paired freshed invasive breast cancer and adjacent normal breast tissues were assessed with real time fluorescence quantitative PCR and methylation-specific polymerase chain reaction（MSP）,respectively.The correlations between these genetic features and clinical feature or prognosis were analyzed.Results The methylation of SFRP1 promoter in invasive breast cancer was significantly higher than that in adjacent normal breast tissues（P0.05）,furthermore;so was in cancer tissues with axillary lymph node metastasis than that in non-axillary lymph node metastasis（P0.05）.However,no significant correlation was revealed between these methylation and other clinical features or prognosis.SFRP1 expression in breast carcinoma was significantly lower than that in adjacent normal breast tissues（P0.05）.In 23 cases invasive breast cancer tissues with methylation of SFRP1 promoter CpG island,the mRNA expression of SFRP1 was not detected.Conclusion Abnormal methylation of SFRP1 may down-regulate the transcription of mRNA and correlate to the oncogenesis of invasive breast cancer,thus providing effective biomarker for early diagnosis and prognosis of invasive breast cancer.

关 键 词：浸润性乳腺癌 SFRP1 甲基化 实时荧光定量PCR技术 甲基化特异性PCR 

分 类 号：R737.9[医药卫生—肿瘤]