外周血促甲状腺素受体mRNA及甲状腺球蛋白mRNA检测对甲状腺癌的诊断意义  

The diagnostic sinification of peripheral blood TSHR-mRNA and Tg-mRNA test in thyroid cancer

作　　者：张伟峰[1] 杨维良[1] 张东伟[1] 张滨[1] 

机构地区：[1]哈尔滨医科大学第二医院普外科,150086

出　　处：《中国肿瘤临床与康复》2011年第6期492-495,共4页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的通过临床对甲状腺癌患者采用逆转录聚合酶链反应(RT-PCR)检测方法对患者的外周血中促甲状腺素受体mRNA(TSHR-mRNA)和甲状腺球蛋白mRNA(Tg-mRNA)进行检测,分析其在甲状腺癌中的表达,并研究其检测结果对甲状腺癌的诊断意义。方法研究对象均选自哈尔滨医科大学第二医院甲状腺外科住院患者,共170例,分正常对照组(30例);甲状腺良性疾病组(40例);甲状腺癌组(100例)。对三组患者分别采用RT-PCR检测方法,比较三组患者的Tg-mRNA、TSHR-mRNA的水平以及临床表达,分析TSHR-mRNA和Tg-mRNA在甲状腺癌中的诊断意义。结果甲状腺癌组患者的TSHR-mRNA以及Tg-mRNA阳性率明显高于正常对照组和甲状腺良性疾病组,差异有统计学意义(P<0.05)。正常甲状腺疾病患者的TSHR-mRNA以及Tg-mRNA水平变化与正常对照组差异无备统计学意义(P>0.05)。结论促TSHR-mRNA以及Tg-mRNA能够准确的反映甲状腺患者的病情。Objective Through the RT-PCR determination methods to analysed the expression of TSHR-mRNA and Tg-mRNA in peripheral blood of thyroid cancer patients, and to evaluated its signification. Methods 170 cases of thyroid surgery hospital ization were divided into 3 groups ： 30 cases for normal control group ; 40 cases for benign thyroid disease, and 100 cases of thyroid cancer. Three groups of patients were used RT-PCR detection method respectively to determinate the Tg-mRNA,TSHR-mRNA level and to evaluate its signification. Results In the group of thyroid cancer, the positive rate of TSHR-mRNA and TgmRNA was higher remarkably than control group and benign group, the difference had statistically siniffcant （P 〈 0. 05 ）. Among the benign thyroid disease group and control group were no statistically significant（ P 〉 0. 05）. Conclusion Through this study we found the peripheral blood of thyroid hormone receptor-promoting mRNA and thyroid protein-mRNA in thyroid cancer patient, the positive expression was higher than oth- er two group patients（ benign and control）. So that the thyroid hormone receptor-mRNA and thyroid protein- mRNA in the clinical diagnosis can accurately reflects the condition of thyroid disease patients.

关 键 词：受体 促甲状腺素 甲状腺球蛋白 RNA 信使 甲状腺肿瘤 诊断 

分 类 号：R736.1[医药卫生—肿瘤] R730.45[医药卫生—临床医学]