槲皮素对人结肠癌SW480细胞增殖的抑制作用  

Research on the inhibitory effect of quercetin on the growth and proliferation in human colon carcinoma cell line SW480 in vitro

作　　者：林增海[1] 马涛[1] 孟勇[1] 

机构地区：[1]汕头大学医学院第一附属医院肝胆外科,515041

出　　处：《实用医学杂志》2012年第5期699-701,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2009B030801321);广东省中医药局科研课题(编号:2009232;2008164)

摘　　要：目的:观察槲皮素对体外培养的人结肠癌SW480细胞的增殖抑制作用。方法:观察不同浓度(5、10、20、40、80、160μmol/L)的槲皮素及50mg/L的5-Fu(阳性对照组)在不同时间(24、48、72h)对SW480细胞增殖的影响。运用细胞计数试剂盒检测法(CCK-8)检测槲皮素对SW480细胞生长的影响;光学显微镜和电子显微镜下观察槲皮素处理后SW480细胞形态结构的变化;碘化丙啶(PI)染色流式细胞术(FCM)分析槲皮素对SW480细胞的细胞周期分布及凋亡率的影响。结果:CCK-8法检测显示槲皮素对SW480细胞的增殖有抑制作用,其抑制作用随着作用浓度的增加和作用时间的延长而增强(P<0.05);经槲皮素处理的SW480细胞与阳性对照组比较,呈明显凋亡状态;PI染色FCM分析发现槲皮素以浓度依赖方式诱导SW480细胞G2/M期细胞累积,且细胞凋亡率随药物浓度增加而增加(P<0.05)。结论:槲皮素对人结肠癌SW480细胞的增殖有抑制作用,且能诱导SW480细胞凋亡,是一种高效低毒的药物。Objective To observe the inhibitory effect on the growth and proliferation of human colon carcinoma SW480 cell line in vitro induced by quercetin. Methods SW480 cells were incubated in vitro by cell biology technique. By means of CCK-8, the effect of quercetin on SW480 cell was studied, the morphological change of SW480 cell was investigated by light microscope and electron microscope. Flow cytometry using propidium iodide （PI） staining was used to determine the cell cycle and cell apoptotic rate. Results Quercetin inhibited the growth of SW480 cell by a time- and dose- dependent manner （P 0.05）. The amount of SW480 cell treated by quercetin decreased. Apoptotic corpuscles were found by electron microscope. The apoptosis rate of SW480 cell was improved, and induced cell cycle arrest in G2/M in does-dependent manner of quercetin （P 0.05）. Conclusion Quercetin can inhibit the growth of SW480 cell by a time- and dose- dependent manner and induce the apoptosis of SW480 cell in vitro.

关 键 词：结肠肿瘤 槲皮素 SW480细胞 增殖 

分 类 号：R735.35[医药卫生—肿瘤]