EGCG诱导人胶质瘤细胞U251凋亡及对survivin表达的影响  

Induction of Apoptosis in Glioblastoma Cell line U251 of Epigallocatechin-3-gallate and its Influence on the Expressions of Surviv in Protein

作　　者：赵志远 谢瑞[2] 王宇[2] 

机构地区：[1]阳泉市第一人民医院,045000 [2]长治医学院病理学教研室

出　　处：《长治医学院学报》2012年第1期16-18,共3页Journal of Changzhi Medical College

摘　　要：目的:观察表没食子儿茶素没食子酸酯(EGCG)诱导人胶质瘤细胞(U251)凋亡的生物学效应及对survivin蛋白表达的影响。方法:采用MTT法检测不同浓度EGCG对U251细胞增殖抑制作用;吖啶橙/溴化乙啶(AO/EB)荧光双染法观察各组细胞凋亡形态,计算凋亡率;Wester Blot法检测不同剂量EGCG处理48h对survivin蛋白表达的影响。结果:EGCG显著抑制U251细胞生长,呈浓度依赖性;EGCG可以诱导U251细胞凋亡,细胞凋亡率随着药物浓度的增加而逐渐增加。Wester Blot结果显示EGCG抑制Survivin蛋白的表达。结论:EGCG具有诱导U251细胞凋亡的作用,其机制可能与抑制Sur-vivin蛋白的表达相关。Objective： To investigate the effect of epigallocatechin-3-gallate on Induction of apoptosis in glioblastoma cell line U251 and to study EGCG＇s influence on the expressions of Survivin protein. Methods .. Ce｜l proliferation was examined by MTT assay in U251 cells treated with different concentrations of EGCG. The apoptosis of U251 cells was determined by AO/EB fluorescence stain. Western Blot analysis was performed to detect the expression of Survivin protein. Results..EGCG inhibited the proliferation of U251 cells in a dose-dependent manner. AO/EB fluorescence stain data provided evidence that the apoptosis of U251 cells induced by EGCG in a dose-dependent manner. Western Blot analysis showed that the expression of Survivin protein were effectively inhibited after EGCG was used. Conclusion：EGCG posseses antitumor effect in vitro,its mechanism might be associated with blocking Survivin expressions.

关 键 词：人胶质瘤 表没食子儿茶素没食子酸酯 SURVIVIN蛋白 凋亡 

分 类 号：R739[医药卫生—肿瘤]