外周血CD19^+ B细胞PD-L1的表达与系统性红斑狼疮发病的相关性分析  

Reduced expression of PD-L1 on CD19^+ B cells from the peripheral blood of patients with systemic lupus erythematosus

作　　者：谢长好[1] 王元元[2] 陈静[1] 肖燕秋[3] 李志军[1] 李柏青[4] 

机构地区：[1]蚌埠医学院第一附属医院风湿免疫科,蚌埠233004 [2]蚌埠医学院组织与胚胎学教研室,蚌埠233030 [3]蚌埠医学院图书馆,蚌埠233030 [4]蚌埠医学院感染与免疫省重点实验室,蚌埠233030

出　　处：《中国免疫学杂志》2012年第2期161-164,167,共5页Chinese Journal of Immunology

基　　金：安徽省卫生厅青年基金(09B108)资助

摘　　要：目的:探讨程序性死亡配体1(Programmed death ligand-1,PD-L1)在系统性红斑狼疮(Systemic lupus erythema-tosus,SLE)患者外周血B细胞上的表达及临床意义。方法:应用流式细胞仪检测51例SLE患者和38例健康对照者外周血CD19+B细胞表面PD-L1的表达水平,比较SLE稳定组、活动组和健康对照组以及狼疮肾炎组和无狼疮肾炎组之间CD19+B细胞表面PD-L1表达阳性细胞的百分比,并分析其与临床表现及实验室检查数据的相关性。结果:SLE活动组和稳定组CD19+PD-L1+B细胞百分率均低于健康对照组,活动组又低于稳定组,差异均有统计学意义(均P<0.05)。狼疮肾炎患者CD19+PD-L1+B细胞百分率低于无狼疮肾炎患者(P<0.05)。SLE患者CD19+PD-L1+B细胞百分率与SLEDAI评分、尿蛋白定量、呈负相关,与C3呈正相关。SLE患者中抗dsDNA抗体、抗Sm抗体、抗U1snRNP抗体、抗核小体抗体阳性组外周血B细胞PD-L1表达水平均低于对应阴性组,且均有统计学意义(均P<0.05)。结论:SLE患者外周血CD19+B细胞表达PD-L1下降,与病情活动性和抗体产生有很好的相关性。Objective：To detect expression of PD-L1 on CD19＋ B cells from patients with systemic lupus erythematosus（SLE） and analyze the clinical relevance to disease severity.Methods：The expression of PD-L1 on the CD19＋ B cells was examined in 51 active SLE patients and 38 healthy controls（HC） by flow cytometry.The proportions of PD-L1 positive CD19＋ B cells were compared between not only inactive or active SLE patients and healthy controls（HC）,but also between patients with and without lupus nephritis.Correlations with clinical manifestations and laboratory findings was analyzed.Results：The Proportions of CD19＋PD-L1＋ B cells were significantly decreased in active and inactive SLE patients as compared with HC（all P0.05）.The number of CD19＋PD-L1＋ B cells in SLE patients with nephritis were significantly lower than those in patients without nephritis.A negative correlation was observed for proportions of CD19＋PD-L1＋B cells with SLEDAI score and amounts of proteinuria.But positively correlated with C3.Reduced numbers of CD19＋PD-L1＋B cells in SLE patients were also correlated to increased level of anti-dsDNA,anti-Sm,anti-U1snRNP and anti-Nucleosome antibodies in SLE patients（all P0.05）.Conclusion：The aberrations of CD19＋PD-L1＋ B cells were observed in patients with SLE.Reduced numbers of CD19＋PD-L1＋B cells were correlated with increased disease activity and the production of anti-self antibodies.

关 键 词：系统性红斑狼疮 PD-L1 B细胞 

分 类 号：R593.24[医药卫生—内科学]